Number of the records: 1
Cytokinins Are Initial Targets of Light in the Control of Bud Outgrowth(1[OPEN])
- 1.
SYSNO ASEP 0468498 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cytokinins Are Initial Targets of Light in the Control of Bud Outgrowth(1[OPEN]) Author(s) Roman, H. (FR)
Girault, T. (FR)
Barbier, F. (FR)
Peron, T. (FR)
Brouard, N. (FR)
Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Vian, A. (FR)
Sakr, S. (FR)
Lothier, J. (FR)
Le Gourrierec, J. (FR)
Leduc, N. (BE)Number of authors 12 Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 172, č. 1 (2016), s. 489-509Number of pages 21 s. Language eng - English Country US - United States Keywords cell-cycle regulation ; pea axillary buds ; oryza-sativa l. ; apical dominance ; rosa sp ; arabidopsis-thaliana ; lateral buds ; meristem activity ; plant-responses ; acts downstream Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000388451900037 DOI 10.1104/pp.16.00530 Annotation Bud outgrowth is controlled by environmental and endogenous factors. Through the use of the photosynthesis inhibitor norflurazon and of masking experiments, evidence is given here that light acts mainly as a morphogenic signal in the triggering of bud outgrowth and that initial steps in the light signaling pathway involve cytokinins (CKs). Indeed, in rose (Rosa hybrida), inhibition of bud outgrowth by darkness is suppressed solely by the application of CKs. In contrast, application of sugars has a limited effect. Exposure of plants to white light (WL) induces a rapid (after 3-6 h of WL exposure) up-regulation of CK synthesis (RhIPT3 and RhIPT5), of CK activation (RhLOG8), and of CK putative transporter RhPUP5 genes and to the repression of the CK degradation RhCKX1 gene in the node. This leads to the accumulation of CKs in the node within 6 h and in the bud at 24 h and to the triggering of bud outgrowth. Molecular analysis of genes involved in major mechanisms of bud outgrowth (strigolactone signaling [RwMAX2], metabolism and transport of auxin [RhPIN1, RhYUC1, and RhTAR1], regulation of sugar sink strength [RhVI, RhSUSY, RhSUC2, and RhSWEET10], and cell division and expansion [RhEXP and RhPCNA]) reveal that, when supplied in darkness, CKs up-regulate their expression as rapidly and as intensely as WL. Additionally, up-regulation of CKs by WL promotes xylem flux toward the bud, as evidenced by Methylene Blue accumulation in the bud after CK treatment in the dark. Altogether, these results suggest that CKs are initial components of the light signaling pathway that controls the initiation of bud outgrowth. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
Number of the records: 1