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Molecular Cytogenetic Mapping of Satellite DNA Sequences in Aegilops geniculata and Wheat

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    SYSNO ASEP0467787
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleMolecular Cytogenetic Mapping of Satellite DNA Sequences in Aegilops geniculata and Wheat
    Author(s) Koo, D.H. (US)
    Tiwari, V.K. (US)
    Hřibová, Eva (UEB-Q) RID, ORCID
    Doležel, Jaroslav (UEB-Q) RID, ORCID
    Friebe, B. (US)
    Gill, B.S. (US)
    Number of authors6
    Source TitleCytogenetic and Genome Research - ISSN 1424-8581
    Roč. 148, č. 4 (2016), s. 314-321
    Number of pages8 s.
    Languageeng - English
    CountryCH - Switzerland
    Keywordsin-situ hybridization ; chromosome addition lines ; resistance genes lr57 ; repetitive dna ; triticum-ovatum ; powdery mildew ; plant genome ; bread wheat ; leaf rust ; identification ; Aegilops geniculata ; Chromosome identification ; Fluorescence in situ hybridization ; Satellite DNA ; Wheat
    Subject RIVEB - Genetics ; Molecular Biology
    R&D ProjectsLO1204 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportUEB-Q - RVO:61389030
    UT WOS000383706100010
    AnnotationFluorescence in situ hybridization (FISH) provides an efficient system for cytogenetic analysis of wild relatives of wheat for individual chromosome identification, elucidation of homoeologous relationships, and for monitoring alien gene transfers into wheat. This study is aimed at developing cytogenetic markers for chromosome identification of wheat and Aegilops geniculata (2n = 4x = 28, (UUMMg)-U-g-M-g-M-g) using satellite DNAs obtained from flow-sorted chromosome 5M(g). FISH was performed to localize the satellite DNAs on chromosomes of wheat and selected Aegilops species. The FISH signals for satellite DNAs on chromosome 5M(g) were generally associated with constitutive heterochromatin regions corresponding to C-band-positive chromatin including telomeric, pericentromeric, centromeric, and interstitial regions of all the 14 chromosome pairs of Ae. geniculata. Most satellite DNAs also generated FISH signals on wheat chromosomes and provided diagnostic chromosome arm-specific cytogenetic markers that significantly improved chromosome identification in wheat. The newly identified satellite DNA CL36 produced localized M-g genome chromosome-specific FISH signals in Ae. geniculata and in the M genome of the putative diploid donor species Ae. comosa subsp. sub-ventricosa but not in Ae. comosa subsp. comosa, suggesting that the M-g genome of Ae. geniculata was probably derived from subsp. subventricosa.
    WorkplaceInstitute of Experimental Botany
    ContactDavid Klier,, Tel.: 220 390 469
    Year of Publishing2017
Number of the records: 1