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Auxin-cytokinin interaction and variations in their metabolic products in the regulation of organogenesis in two Eucomis species
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SYSNO ASEP 0465915 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Auxin-cytokinin interaction and variations in their metabolic products in the regulation of organogenesis in two Eucomis species Author(s) Aremu, A. O. (ZA)
Plačková, Lenka (UEB-Q) ORCID, RID
Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Doležal, Karel (UEB-Q) RID, ORCID
Van Staden, J. (ZA)Number of authors 6 Source Title New Biotechnology. - : Elsevier - ISSN 1871-6784
Roč. 33, č. 6 (2016), s. 883-890Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords in-vitro propagation ; plant-growth regulators ; arabidopsis-thaliana ; quantitative-analysis ; mass-spectrometry ; isotope-dilution ; biosynthesis ; gradients ; cultures ; hormones ; Asparagaceae ; Indole-3-acetic acid ; Phytohormones ; meta-Topolin ; Ornamentals ; Rooting Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA14-34792S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000386782600016 DOI 10.1016/j.nbt.2016.09.001 Annotation In the current study, we evaluated the effect of alpha-naphthaleneacetic acid (NAA) individually or in combination with different cytokinins (CKs) including benzyladenine (BA), meta-topolin (mT) and isopentenyladenine (iP) on organogenesis, auxin and CK content in Eucomis autumnalis subspecies autumnalis (EA) and Eucomis zambesiaca (EZ). These species were used as model plants due to their ornamental and medicinal properties. Three leaf explants were inoculated in screw-cap jars containing 30 mL Murashige and Skoog (MS) media supplemented with 5 mM NAA alone or in combination with 5 mu M CK (BA, mT or iP). After 10 weeks (EA) or 15 weeks (EZ), parameters including shoot and root growth as well as plant fresh weight were recorded. For analysis of auxin and CK content, whole plantlets were harvested, pooled and freeze-dried for the different treatments. In both species, shoot and root proliferation as well as plant biomass were generally higher when NAA was combined with the individual CK than in NAA or CK treatment. The highest concentration of indole-3-acetic acid (IAA, 619 pmol g(-1) DW) and 2-oxindole-3-acetic acid (OxIAA, 2381 pmol g(-1) DW) were observed in EA-treated with NAA alone while mT treatment (without NAA) had the most abundant indole-3-acetyl-L-aspartic acid (IAAsp, 904 and 582 pmol g(-1) DW for EA and EZ, respectively) in both species. A significant concentration of total endogenous CK accumulated in both Eucomis regenerants from mT and mT + NAA when compared to the other treatments. The majority of the detected CKs were of the aromatic CK-type, mainly free bases. The potential physiological roles of these quantified phytohormones in relation to the observed morphological responses are discussed. (C) 2016 Elsevier B.V. All rights reserved. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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