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Rapid gene isolation in barley and wheat by mutant chromosome sequencing
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SYSNO ASEP 0465513 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Rapid gene isolation in barley and wheat by mutant chromosome sequencing Author(s) Sanchez-Martin, J. (CH)
Steuernagel, B. (GB)
Ghosh, S. (GB)
Herren, G. (CH)
Hurni, S. (CH)
Adamski, N. (GB)
Vrána, Jan (UEB-Q) RID, ORCID
Kubaláková, Marie (UEB-Q) RID
Krattinger, S.G. (CH)
Wicker, T. (CH)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Keller, B. (CH)
Wulff, B. B. H. (GB)Number of authors 13 Article number 221 Source Title Genome Biology - ISSN 1465-6906
Roč. 17, OCT 31 (2016)Number of pages 7 s. Language eng - English Country US - United States Keywords induced mutations ; mitotic chromosomes ; confers resistance ; exome capture ; genome ; identification ; evolution ; pathogens ; hordeum ; MutChromSeq ; Gene cloning ; Mutational genomics ; Chromosome flow sorting ; Triticeae ; Wheat ; Barley Subject RIV EB - Genetics ; Molecular Biology R&D Projects GBP501/12/G090 GA ČR - Czech Science Foundation (CSF) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000386392200001 DOI 10.1186/s13059-016-1082-1 Annotation Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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