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Separation and identification of candidate protein elicitors from the cultivation medium of Leptosphaeria maculans inducing resistance in Brassica napus
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SYSNO ASEP 0464107 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Separation and identification of candidate protein elicitors from the cultivation medium of Leptosphaeria maculans inducing resistance in Brassica napus Author(s) Nováková, Miroslava (UEB-Q) RID
Kim, P.D. (CZ)
Šašek, Vladimír (UEB-Q) RID
Burketová, Lenka (UEB-Q) RID, ORCID
Jindřichová, Barbora (UEB-Q) RID, ORCID
Šantrůček, J. (CZ)
Valentová, O. (CZ)Source Title Biotechnology Progress. - : Wiley - ISSN 8756-7938
Roč. 32, č. 4 (2016), s. 918-928Number of pages 11 s. Language eng - English Country US - United States Keywords elicitor ; Brassica napus ; Leptosphaeria maculans Subject RIV GF - Plant Pathology, Vermin, Weed, Plant Protection R&D Projects GA522/08/1581 GA ČR - Czech Science Foundation (CSF) QH81201 GA MZe - Ministry of Agriculture (MZe) LD14093 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000383396800011 DOI 10.1002/btpr.2266 Annotation The Dothideomycete Leptosphaeria maculans, a worldwide fungal pathogen of oilseed rape (Brassica napus), secretes a broad spectrum of molecules into the cultivation medium during growth in vitro. Here, candidate elicitor molecules, which induce resistance in B. napus to L. maculans, were identified in the cultivation medium. The elicitation activity was indicated by increased transcription of pathogenesis-related gene 1 (PR1) and enhanced resistance of B. napus plants to the invasion of L. maculans. The elicitation activity was significantly lowered when the cultivation medium was heated to 80 degrees C. Active components were further characterized by specific cleavage with the proteolytic enzymes trypsin and proteinase K and with glycosidases -amylase and -glucanase. The elicitor activity was eliminated by proteolytic digestion while glycosidases had no effect. The filtered medium was fractionated by either ion-exchange chromatography or isoelectric focusing. Mass spectrometry analysis of the most active fractions obtained by both separation procedures revealed predominantly enzymes that can be involved in the degradation of plant cell wall polysaccharides. This is the first study searching for L. maculans-specific secreted elicitors with a potential to be used as defense-activating agents in the protection of B. napus against L. maculans in agriculture. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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