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Function of the Golgi-located phosphate transporter PHT4;6 is critical for senescence-associated processes in Arabidopsis
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SYSNO ASEP 0464106 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Function of the Golgi-located phosphate transporter PHT4;6 is critical for senescence-associated processes in Arabidopsis Author(s) Hassler, S. (DE)
Jung, B. (DE)
Lemke, L. (DE)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Strnad, Miroslav (UEB-Q) RID, ORCID
Martinoia, E. (CH)
Neuhaus, H.E. (DE)Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 67, č. 15 (2016), s. 4671-4684Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords ammonium ; cytokinin ; golgi Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA15-22322S GA ČR - Czech Science Foundation (CSF) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000383233400021 DOI 10.1093/jxb/erw249 Annotation Plants with altered intracellular Pi compartmentation surprisingly show accelerated dark-induced senescence. We propose that low cytosolic phosphate concentrations are of critical importance for proper plant development.The phosphate transporter and its impaired activity causes altered intracellular phosphate compartmentation, leading to low cytosolic Pi levels, a blockage of Golgi-related processes such as protein glycosylation and hemicellulose biosynthesis, and a dwarf phenotype. However, it was unclear whether altered Pi homeostasis in pht4;6 mutants causes further cellular problems, typically associated with limited phosphate availability. Here we report that pht4;6 mutants exhibit a markedly increased disposition to induce dark-induced senescence. In control experiments, in which pht4;6 mutants and wild-type plants developed similarly, we confirmed that accelerated dark-induced senescence in mutants is not a 'pleiotropic' process associated with the dwarf phenotype. In fact, accelerated dark-induced senescence in pht4;6 mutants correlates strongly with increased levels of toxic NH4 (+) and higher sensitivity to ammonium, which probably contribute to the inability of pht4;6 mutants to recover from dark treatment. Experiments with modified levels of either salicylic acid (SA) or trans-zeatin (tZ) demonstrate that altered concentrations of these compounds in pht4;6 plants act as major cellular mediators for dark-induced senescence. This conclusion gained further support from the notion that the expression of the pht4;6 gene is, in contrast to genes coding for major phosphate importers, substantially induced by tZ. Taken together, our findings point to a critical function of PHT4;6 to control cellular phosphate levels, in particular the cytosolic Pi availability, required to energize plant primary metabolism for proper plant development. Phosphate and its allocation mediated by PHT4;6 is critical to prevent onset of dark-induced senescence. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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