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The DAG1 transcription factor negatively regulates the seed-to-seedling transition in Arabidopsis acting on ABA and GA levels

SYSNO ASEP	0463854
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The DAG1 transcription factor negatively regulates the seed-to-seedling transition in Arabidopsis acting on ABA and GA levels
Author(s)	Boccaccini, A. (GB) Lorrai, R. (IT) Ruta, V. (IT) Frey, A. (FR) Mercey-Boutet, S. (FR) Marion-Poll, F. (FR) Tarkowská, Danuše (UEB-Q)_{RID, ORCID} Strnad, Miroslav (UEB-Q)_{RID, ORCID} Costantino, P. (IT) Vittorioso, P. (IT)
Source Title	BMC Plant Biology. - : BioMed Central - ISSN 1471-2229 Roč. 16, SEP 9 (2016), s. 198
Number of pages	11 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	DAG1 ; Seed development ; Chromatin remodelling
Subject RIV	EF - Botanics
R&D Projects	LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA14-34792S GA ČR - Czech Science Foundation (CSF)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000382908700001
DOI	10.1186/s12870-016-0890-5
Annotation	Background: In seeds, the transition from dormancy to germination is regulated by abscisic acid (ABA) and gibberellins (GAs), and involves chromatin remodelling. Particularly, the repressive mark H3K27 trimethylation (H3K27me3) has been shown to target many master regulators of this transition. DAG1 (DOF AFFECTING GERMINATION1), is a negative regulator of seed germination in Arabidopsis, and directly represses the GA biosynthetic gene GA3ox1 (gibberellin 3-beta-dioxygenase 1). We set to investigate the role of DAG1 in seed dormancy and maturation with respect to epigenetic and hormonal control. Results: We show that DAG1 expression is controlled at the epigenetic level through the H3K27me3 mark during the seed-to-seedling transition, and that DAG1 directly represses also the ABA catabolic gene CYP707A2; consistently, the ABA level is lower while the GA level is higher in dag1 mutant seeds. Furthermore, both DAG1 expression and protein stability are controlled by GAs. Conclusions: Our results point to DAG1 as a key player in the control of the developmental switch between seed dormancy and germination.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2017

Number of the records: 1