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The wheat Phs-A1 pre-harvest sprouting resistance locus delays the rate of seed dormancy loss and maps 0.3 cM distal to the PM19 genes in UK germplasm
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SYSNO ASEP 0463800 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The wheat Phs-A1 pre-harvest sprouting resistance locus delays the rate of seed dormancy loss and maps 0.3 cM distal to the PM19 genes in UK germplasm Author(s) Shorinola, O. (GB)
Bird, N. (GB)
Simmonds, J. (GB)
Berry, S. (US)
Henriksson, T. (SE)
Jack, P. (GB)
Werner, P. (DE)
Gerjets, T. (GB)
Scholefield, D. (GB)
Balcárková, Barbora (UEB-Q)
Valárik, Miroslav (UEB-Q) RID, ORCID
Holdsworth, M.J. (GB)
Flintham, J. (GB)
Uauy, C. (GB)Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 67, č. 14 (2016), s. 4169-4178Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords After-ripening ; dormancy ; PM19 Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA14-07164S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000382060300014 DOI 10.1093/jxb/erw194 Annotation Phs-A1 confers resistance to sprouting in wheat by delaying the rate of seed dormancy loss and is distinct from the previously proposed PM19 candidate genes.The precocious germination of cereal grains before harvest, also known as pre-harvest sprouting, is an important source of yield and quality loss in cereal production. Pre-harvest sprouting is a complex grain defect and is becoming an increasing challenge due to changing climate patterns. Resistance to sprouting is multi-genic, although a significant proportion of the sprouting variation in modern wheat cultivars is controlled by a few major quantitative trait loci, including Phs-A1 in chromosome arm 4AL. Despite its importance, little is known about the physiological basis and the gene(s) underlying this important locus. In this study, we characterized Phs-A1 and show that it confers resistance to sprouting damage by affecting the rate of dormancy loss during dry seed after-ripening. We show Phs-A1 to be effective even when seeds develop at low temperature (13 A degrees C). Comparative analysis of syntenic Phs-A1 intervals in wheat and Brachypodium uncovered ten orthologous genes, including the Plasma Membrane 19 genes (PM19-A1 and PM19-A2) previously proposed as the main candidates for this locus. However, high-resolution fine-mapping in two bi-parental UK mapping populations delimited Phs-A1 to an interval 0.3 cM distal to the PM19 genes. This study suggests the possibility that more than one causal gene underlies this major pre-harvest sprouting locus. The information and resources reported in this study will help test this hypothesis across a wider set of germplasm and will be of importance for breeding more sprouting resilient wheat varieties. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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