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Leptosphaeria maculans effector AvrLm4-7 affects salicylic acid (SA) and ethylene (ET) signalling and hydrogen peroxide (H2O2) accumulation in Brassica napus

SYSNO ASEP	0462768
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Leptosphaeria maculans effector AvrLm4-7 affects salicylic acid (SA) and ethylene (ET) signalling and hydrogen peroxide (H2O2) accumulation in Brassica napus
Author(s)	Nováková, Miroslava (UEB-Q)_RID Šašek, Vladimír (UEB-Q)_RID Trdá, Lucie (UEB-Q)_ORCID Krutinová, Hana (UEB-Q) Mongin, T. (FR) Valentová, O. (CZ) Balesdent, M.H. (FR) Rouxel, T. (FR) Burketová, Lenka (UEB-Q)_{RID, ORCID}
Source Title	Molecular Plant Pathology. - : Wiley - ISSN 1464-6722 Roč. 17, č. 6 (2016), s. 818-831
Number of pages	15 s.
Language	eng - English
Country	US - United States
Keywords	AvrLm4-7 ; Brassica napus ; effector
Subject RIV	GF - Plant Pathology, Vermin, Weed, Plant Protection
R&D Projects	GA13-26798S GA ČR - Czech Science Foundation (CSF)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000379948300004
DOI	10.1111/mpp.12332
Annotation	To achieve host colonization, successful pathogens need to overcome plant basal defences. For this, (hemi)biotrophic pathogens secrete effectors that interfere with a range of physiological processes of the host plant. AvrLm4-7 is one of the cloned effectors from the hemibiotrophic fungus Leptosphaeria maculans brassicaceae' infecting mainly oilseed rape (Brassica napus). Although its mode of action is still unknown, AvrLm4-7 is strongly involved in L. maculans virulence. Here, we investigated the effect of AvrLm4-7 on plant defence responses in a susceptible cultivar of B. napus. Using two isogenic L. maculans isolates differing in the presence of a functional AvrLm4-7 allele [absence (a4a7') and presence (A4A7') of the allele], the plant hormone concentrations, defence-related gene transcription and reactive oxygen species (ROS) accumulation were analysed in infected B. napus cotyledons. Various components of the plant immune system were affected. Infection with the A4A7' isolate caused suppression of salicylic acid- and ethylene-dependent signalling, the pathways regulating an effective defence against L. maculans infection. Furthermore, ROS accumulation was decreased in cotyledons infected with the A4A7' isolate. Treatment with an antioxidant agent, ascorbic acid, increased the aggressiveness of the a4a7' L. maculans isolate, but not that of the A4A7' isolate. Together, our results suggest that the increased aggressiveness of the A4A7' L. maculans isolate could be caused by defects in ROS-dependent defence and/or linked to suppressed SA and ET signalling. This is the first study to provide insights into the manipulation of B. napus defence responses by an effector of L. maculans.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2017

Number of the records: 1