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Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia)
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SYSNO ASEP 0461920 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia) Author(s) Stuchlíková, L. (CZ)
Jirásko, R. (CZ)
Skálová, L. (CZ)
Pavlík, F. (CZ)
Szotáková, B. (CZ)
Holčapek, M. (CZ)
Vaněk, Tomáš (UEB-Q) RID, ORCID
Podlipná, Radka (UEB-Q) RID, ORCIDSource Title Chemosphere. - : Elsevier - ISSN 0045-6535
Roč. 157, AUG (2016), s. 10-17Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords Drug metabolism ; Biotransformation ; Albendazole Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA15-05325S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000378452400002 DOI 10.1016/j.chemosphere.2016.05.015 Annotation Benzimidazoles anthelmintics, which enter into environment primarily through excretion in the feces or urine of treated animals, can affect various organisms and disrupt ecosystem balance. The present study was designed to test the phytotoxicity and biotransformation of the three benzimidazole anthelmintics albendazole (ABZ), fenbendazole (FBZ) and flubendazole (FLU) in the harebell (Campanula rotundifolia). This meadow plant commonly grows in pastures and comes into contact with anthelmintics through the excrements of treated animals. Suspensions of harebell cells in culture medium were used as an in vitro model system. ABZ, FLU and FBZ were not found to be toxic for harebell cells, which were able to metabolize ABZ, FLU and FBZ via the formation of a wide scale of metabolites. Ultrahigh-performance liquid chromatography coupled with high mass accuracy tandem mass spectrometry (UHPLC-MS/MS) led to the identification of 24, 18 and 29 metabolites of ABZ, FLU and FBZ, respectively. Several novel metabolites were identified for the first time. Based on the obtained results, the schemes of the metabolic pathways of these anthelmintics were proposed. Most of these metabolites can be considered deactivation products, but a substantial portion of them may readily be decomposed to biologically active substances which could negatively affect ecosystems. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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