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Transgenic barley overexpressing a cytokinin dehydrogenase gene shows greater tolerance to drought stress
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SYSNO ASEP 0461675 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transgenic barley overexpressing a cytokinin dehydrogenase gene shows greater tolerance to drought stress Author(s) Pospíšilová, H. (CZ)
Jiskrová, E. (CZ)
Vojta, P. (CZ)
Mrízová, K. (CZ)
Kokáš, F. (CZ)
Majeská Čudějková, M. (CZ)
Bergougnoux, V. (CZ)
Plíhal, O. (CZ)
Klimešová, J. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Dzurová, L. (CZ)
Frébort, I. (CZ)
Galuszka, P. (CZ)Source Title New Biotechnology. - : Elsevier - ISSN 1871-6784
Roč. 33, č. 5 (2016), s. 692-705Number of pages 14 s. Language eng - English Country NL - Netherlands Keywords ROOT-GROWTH ; OXIDASE/DEHYDROGENASE GENES ; BETA-GLUCOSIDASE Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000378026400011 DOI 10.1016/j.nbt.2015.12.005 Annotation Together with auxins, cytokinins are the main plant hormones involved in many different physiological processes. Given this knowledge, cytokinin levels can be manipulated by genetic modification in order to improve agronomic parameters of cereals in relation to, for example, morphology, yield, and tolerance to various stresses. The barley (Hordeum vulgare) cultivar Golden Promise was transformed using the cytokinin dehydrogenase 1 gene from Arabidopsis thaliana (AtCKX1) under the control of mild root-specific beta-glucosidase promoter from maize. Increased cytokinin degradation activity was observed positively to affect the number and length of lateral roots. The impact on morphology depended upon the recombinant protein's subcellular compartmentation. While assumed cytosolic and vacuolar targeting of AtCKX1 had negligible effect on shoot growth, secretion of AtCKX1 protein to the apoplast had a negative effect on development of the aerial part and yield. Upon the application of severe drought stress, all transgenic genotypes maintained higher water content and showed better growth and yield parameters during revitalization. Higher tolerance to drought stress was most caused by altered root morphology resulting in better dehydration avoidance. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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