Number of the records: 1

Cytokinin, auxin and physiological polarity in the aquatic carnivorous plants Aldrovanda vesiculosa and Utricularia australis

SYSNO ASEP	0461643
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cytokinin, auxin and physiological polarity in the aquatic carnivorous plants Aldrovanda vesiculosa and Utricularia australis
Author(s)	Šimura, Jan (UEB-Q)_{ORCID, RID} Spíchal, Lukáš (UEB-Q)_{RID, ORCID} Adamec, Lubomír (BU-J)_{RID, ORCID} Pěnčík, A. (CZ) Rolčík, Jakub (UEB-Q) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Source Title	Annals of Botany. - : Oxford University Press - ISSN 0305-7364 Roč. 117, č. 6 (2016), s. 1037-1044
Number of pages	8 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Auxin ; Aldrovanda vesiculosa ; cytokinin
Subject RIV	EF - Botanics
OECD category	Plant sciences, botany
R&D Projects	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030 ; BU-J - RVO:67985939
UT WOS	000376655400008
EID SCOPUS	84971554682
DOI	10.1093/aob/mcw020
Annotation	Methods The main auxin and cytokinin metabolites in separated shoot segments and turions of two carnivorous plants, Aldrovanda vesiculosa and Utricularia australis, were analysed using ultra-high-performance liquid chromatography coupled with triple quad mass spectrometry. Key Results In both species, only isoprenoid cytokinins were identified. Zeatin cytokinins predominated in the apical parts, with their concentrations decreasing basipetally, and the trans isomer predominated in A. vesiculosa whereas the cis form was more abundant in U australis. Isopentenyladenine-type cytokinins, in contrast, increased basipetally. Conjugated cytokinin metabolites, the O-glucosides, were present at high concentrations in A. vesiculosa but only in minute amounts in U. australis. N-9-glucoside forms were detected only in U. australis, with isopentenyladenine-9-glucoside (iP9G) being most abundant. In addition to free indole-3-acetic acid (IAA), indole-3-acetamide (IAM), IAA-aspartate (IAAsp), IAA-glutamate (IAGlu) and IAA-glycine (IAGly) conjugates were identified. Conclusions Both species show common trends in auxin and cytokinin levels, the apical localization of the cytokinin biosynthesis and basipetal change in the ratio of active cytokinins to auxin, in favour of auxin. However, our detailed study of cytokinin metabolic profiles also revealed that both species developed different regulatory mechanisms of active cytokinin content; on the level of their degradation, in U. australis, or in the biosynthesis itself, in the case of A. vesiculosa. Results indicate that the rapid turnover of these signalling molecules along the shoots is essential for maintaining the dynamic balance between the rapid polar growth and development of the apical parts and senescence of the older, basal parts of the shoots.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2017

Number of the records: 1