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In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled Siderophores
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SYSNO ASEP 0461642 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled Siderophores Author(s) Petřík, M. (CZ)
Zhai, C. (AT)
Nový, Z. (CZ)
Urbánek, Lubor (UEB-Q) RID
Haas, H. (AT)
Decristoforo, C. (AT)Source Title Molecular Imaging and Biology. - : Springer - ISSN 1536-1632
Roč. 18, č. 3 (2016), s. 344-352Number of pages 9 s. Language eng - English Country US - United States Keywords Siderophores ; Gallium-68 ; Zirconium-89 Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1304 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) TE01020028 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Institutional support UEB-Q - RVO:61389030 UT WOS 000376623600005 DOI 10.1007/s11307-015-0897-6 Annotation Some [Ga-68]siderophores show promise in specific and sensitive imaging of infection. Here, we compare the in vitro and in vivo behaviour of selected Ga-68 and Zr-89 labelled siderophores.
Radiolabelling was performed in HEPES or sodium acetate buffer systems. Radiochemical purity of labelled siderophores was determined using chromatography. Partition coefficients, in vitro stability and protein binding affinities were determined. Ex vivo biodistribution and animal imaging was studied in mice.
Certain differences among studied siderophores were observed in labelling efficiency. Protein binding and stability tests showed highest stabilities and lowest protein binding affinities for Ga-68 and [Zr-89]triacetylfusarinine C (TAFC). All studied Ga-68 and [Zr-89]siderophores exhibited a similar biodistribution and pharmacokinetics in mice with the exception of [Zr-89]ferrioxamine E (FOXE).
Zr-89 and [Ga-68]siderophores showed analogous in vitro and in vivo behaviour. Tested [Zr-89]siderophores could be applied for longitudinal positron emission tomography (PET) studies of fungal infections and especially TAFC for the development of novel bioconjugates.Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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