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Arabidopsis FH1 Formin Affects Cotyledon Pavement Cell Shape by Modulating Cytoskeleton Dynamics
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SYSNO ASEP 0461618 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Arabidopsis FH1 Formin Affects Cotyledon Pavement Cell Shape by Modulating Cytoskeleton Dynamics Author(s) Rosero, A. (CZ)
Oulehlová, Denisa (UEB-Q) RID
Stillerová, L. (CZ)
Schiebertová, P. (CZ)
Gunt, M. (CZ)
Žárský, Viktor (UEB-Q) RID, ORCID
Cvrčková, F. (CZ)Source Title Plant and Cell Physiology. - : Oxford University Press - ISSN 0032-0781
Roč. 57, č. 3 (2016), s. 488-504Number of pages 17 s. Language eng - English Country JP - Japan Keywords Arabidopsis thaliana ; Confocal microscopy ; Cotyledon pavement cells Subject RIV ED - Physiology Institutional support UEB-Q - RVO:61389030 UT WOS 000374572400005 DOI 10.1093/pcp/pcv209 Annotation Plant cell morphogenesis involves concerted rearrangements of microtubules and actin microfilaments. We previously reported that FH1, the main Arabidopsis thaliana housekeeping Class I membrane-anchored formin, contributes to actin dynamics and microtubule stability in rhizodermis cells. Here we examine the effects of mutations affecting FH1 (At3g25500) on cell morphogenesis and above-ground organ development in seedlings, as well as on cytoskeletal organization and dynamics, using a combination of confocal and variable angle epifluorescence microscopy with a pharmacological approach. Homozygous fh1 mutants exhibited cotyledon epinasty and had larger cotyledon pavement cells with more pronounced lobes than the wild type. The pavement cell shape alterations were enhanced by expression of the fluorescent microtubule marker GFP-microtubule-associated protein 4 (MAP4). Mutant cotyledon pavement cells exhibited reduced density and increased stability of microfilament bundles, as well as enhanced dynamics of microtubules. Analogous results were also obtained upon treatments with the formin inhibitor SMIFH2 (small molecule inhibitor of formin homology 2 domains). Pavement cell shape in wild-type (wt) and fh1 plants in some situations exhibited a differential response towards anti-cytoskeletal drugs, especially the microtubule disruptor oryzalin. Our observations indicate that FH1 participates in the control of microtubule dynamics, possibly via its effects on actin, subsequently influencing cell morphogenesis and macroscopic organ development. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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