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Repetitive Inductions of Bioluminescence of Pseudomonas putida TVA8 Immobilized by Adsorption on Optical Fibre.
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SYSNO ASEP 0461501 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Repetitive Inductions of Bioluminescence of Pseudomonas putida TVA8 Immobilized by Adsorption on Optical Fibre. Author(s) Zajíc, J. (CZ)
Bittner, M. (CZ)
Brányik, T. (CZ)
Solovyev, Andrey (UCHP-M) RID, ORCID, SAI
Šabata, Stanislav (UCHP-M) RID, ORCID, SAI
Kuncová, Gabriela (UCHP-M) RID, SAI
Pospíšilová, M. (CZ)Source Title Chemical Papers. - : Springer - ISSN 0366-6352
Roč. 70, č. 7 (2016), s. 877-887Number of pages 11 s. Language eng - English Country SK - Slovakia Keywords whole-cell biosensor ; bioluminiscent bioreporter ; optical fibre sensor Subject RIV CC - Organic Chemistry Institutional support UCHP-M - RVO:67985858 UT WOS 000379757200002 EID SCOPUS 85010288574 DOI 10.1515/chempap-2016-0031 Annotation Physico-chemical models of the interactions of cells with solid surfaces, which use contact angles and zeta potentials, indicated more facile adsorption of cells of Pseudomonas putida TVA8 on the quartz surface after its treatment with 3-aminopropyltriethoxysilane (APTES). A whole-cell optical fibre sensor of toluene was prepared by the adsorption of P. putida TVA8, bacteria producing light in contact with toluene on the wider end of APTES-treated quartz tapered optical fibre. The results of the measurements of luminescence from both sides of the layer of adsorbed cells were compared. Over the 135 days trial, the fibre biosensor was repetitively induced with toluene solution (26.5 mg L-1) 68 times. The intensities of bioluminescence gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of bioluminescence induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene. Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2017
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