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Application of Concanavalin A during immune responsiveness skin-swelling tests facilitates measurement interpretation in mammalian ecology
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SYSNO ASEP 0461399 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Application of Concanavalin A during immune responsiveness skin-swelling tests facilitates measurement interpretation in mammalian ecology Author(s) Bílková, B. (CZ)
Albrecht, Tomáš (UBO-W) RID, SAI, ORCID
Chudíčková, Milada (UEM-P) RID, ORCID
Holáň, Vladimír (UEM-P) RID
Piálek, Jaroslav (UBO-W) RID, ORCID, SAI
Vinkler, M. (CZ)Number of authors 6 Source Title Ecology and Evolution. - : Wiley - ISSN 2045-7758
Roč. 6, č. 13 (2016), s. 4551-4564Number of pages 14 s. Language eng - English Country US - United States Keywords Concanavalin A ; cytokine ; ecoimmunology ; evolutionary immunology ; field immunoecological research ; histology ; leucocytes ; PHA-induced hypersensitivity ; phytohemagglutinin ; T-cell-mediated immunocompetence Subject RIV EG - Zoology R&D Projects GA206/08/0640 GA ČR - Czech Science Foundation (CSF) GA15-11782S GA ČR - Czech Science Foundation (CSF) Institutional support UBO-W - RVO:68081766 ; UEM-P - RVO:68378041 UT WOS 000379344400026 EID SCOPUS 84977516136 DOI 10.1002/ece3.2211 Annotation The skin-swelling test is a simple and widespread method used in field ecological research to estimate cellular immune responsiveness in animals. This immunoecological test is based on measuring the magnitude of tissue swelling response at specific times following subcutaneous application of an experimental pro-inflammatory stimulant. In the vast majority of studies across vertebrate taxa, phytohemagglutinin (PHA) is used as a universal stimulant. Given the complexity of immune response activation pathways of PHA, however, interpretation of test results can be ambiguous. The goal of this study was to improve methodology of the skin-swelling test. Based on previous evidence suggesting that mammalian T cells are readily activated by Concanavalin A (ConA) in vitro, we compared cellular immune responses in vivo to PHA and ConA as an alternative proinflammatory stimulant in mice. Our results corroborate that PHA and ConA show important differences in both dynamics and response amplitude in rodents. ConA induces stronger swelling with a distinct leukocyte activity pattern and higher proinflammatory cytokine and interferon gamma expression than PHA during peak response. We conclude that ConA is the more suitable stimulant for skin-swelling tests in mammals. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2017
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