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A previously undescribed jasmonate compound in flowering Arabidopsis thaliana - The identification of cis-(+)-OPDA-Ile

SYSNO ASEP	0458757
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	A previously undescribed jasmonate compound in flowering Arabidopsis thaliana - The identification of cis-(+)-OPDA-Ile
Author(s)	Floková, K. (CZ) Feussner, K. (DE) Herrfurth, C. (DE) Miersch, O. (CZ) Mik, V. (CZ) Tarkowská, Danuše (UEB-Q)_{RID, ORCID} Strnad, Miroslav (UEB-Q)_{RID, ORCID} Feussner, I. (DE) Wasternack, Claus (UEB-Q)_ORCID Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI}
Source Title	Phytochemistry. - : Elsevier - ISSN 0031-9422 Roč. 122, FEB (2016), s. 230-237
Number of pages	8 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Arabidopsis thaliana (Brassicaceae) ; Jasmonates ; Cis-(+)-12-oxo-phytodienoyl-L-isoleucine
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000369460200025
DOI	10.1016/j.phytochem.2015.11.012
Annotation	Jasmonates (JAs) are plant hormones that integrate external stress stimuli with physiological responses. (+)-7-iso-JA-L-Ile is the natural JA ligand of COI1, a component of a known JA receptor. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-(+)-OPDA) has been reported to act independently of COI1 as an essential signal in several stress-induced and developmental processes. Wound induced increases in the endogenous levels of JA/JA-Ile are accompanied by two to tenfold increases in the concentration of OPDA, but its means of perception and metabolism are unknown. To screen for putative OPDA metabolites, vegetative tissues of flowering Arabidopsis thaliana were extracted with 25% aqueous methanol (v/v), purified by single-step reversed-phase polymer-based solid-phase extraction, and analyzed by high throughput mass spectrometry. This enabled the detection and quantitation of a low abundant OPDA analog of the biologically active (+)-7-iso-JA-L-Ile in plant tissue samples. Levels of the newly identified compound and the related phytohormones JA, JA-Ile and cis-(+)-OPDA were monitored in wounded leaves of flowering Arabidopsis lines (Col-0 and Ws) and compared to the levels observed in Arabidopsis mutants deficient in the biosynthesis of JA (dde2-2, opr3) and JA-Ile (jarl). The observed cis-(+)-OPDA-Ile levels varied widely, raising,questions concerning its role in Arabidopsis stress responses.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2017

Number of the records: 1