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Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis
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SYSNO ASEP 0456183 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis Author(s) Zhang, C. (US)
Brown, M.Q. (US)
van de Ven, W. (US)
Zhang, Z.M. (US)
Wu, B. (US)
Young, M.C. (US)
Synek, Lukáš (UEB-Q) RID, ORCID
Borchardt, D. (US)
Harrison, R. (US)
Pan, S.Q. (US)
Luo, N. (US)
Huang, Y.M.M. (US)
Ghang, Y.J. (US)
Ung, N. (US)
Li, R.X. (US)
Isley, J. (US)
Morikis, D. (US)
Song, J.K. (US)
Guo, W. (US)
Hooley, R.J. (US)
Chang, C.E.A. (US)
Yang, Z.B. (US)
Žárský, Viktor (UEB-Q) RID, ORCID
Muday, G.K. (US)
Hicks, G.R. (US)
Raikhel, N.V. (US)Source Title Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 113, č. 1 (2016), E41-E50Number of pages 10 s. Language eng - English Country US - United States Keywords endosidin2 ; exocytosis ; exocyst Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA15-14886S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000367520400009 DOI 10.1073/pnas.1521248112 Annotation The exocyst complex regulates the last steps of exocytosis, which is essential to organisms across kingdoms. In humans, its dysfunction is correlated with several significant diseases, such as diabetes and cancer progression. Investigation of the dynamic regulation of the evolutionarily conserved exocyst-related processes using mutants in genetically tractable organisms such as Arabidopsis thaliana is limited by the lethality or the severity of phenotypes. We discovered that the small molecule Endosidin2 (ES2) binds to the EXO70 (exocyst component of 70 kDa) subunit of the exocyst complex, resulting in inhibition of exocytosis and endosomal recycling in both plant and human cells and enhancement of plant vacuolar trafficking. An EXO70 protein with a C-terminal truncation results in dominant ES2 resistance, uncovering possible distinct regulatory roles for the N terminus of the protein. This study not only provides a valuable tool in studying exocytosis regulation but also offers a potentially new target for drugs aimed at addressing human disease. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
Number of the records: 1