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Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress

SYSNO ASEP	0451388
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress
Author(s)	Krčková, Zuzana (UEB-Q)_ORCID Brouzdová, Jitka (UEB-Q) Daněk, Michal (UEB-Q)_ORCID Kocourková, Daniela (UEB-Q)_ORCID Rainteau, D. (FR) Ruelland, E. (FR) Valentová, O. (CZ) Pejchar, Přemysl (UEB-Q)_{RID, ORCID} Martinec, Jan (UEB-Q)_{RID, ORCID}
Source Title	Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X Roč. 6, NOV 4 (2015), s. 928
Number of pages	13 s.
Language	eng - English
Country	CH - Switzerland
Keywords	Arabidopsis thaliana ; Diacylglycerol ; Heat stress
Subject RIV	ED - Physiology
R&D Projects	GAP501/12/1942 GA ČR - Czech Science Foundation (CSF)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000364581700001
EID SCOPUS	84947424867
DOI	10.3389/fpls.2015.00928
Annotation	The Arabidopsis non-specific phospholipase C1 (NPC) protein family is encoded by the genes NPC1 – NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2016

Number of the records: 1