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Cytokinin profiling of long-term in vitro pea (Pisum sativum L.) shoot cultures

SYSNO ASEP	0450909
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cytokinin profiling of long-term in vitro pea (Pisum sativum L.) shoot cultures
Author(s)	Plačková, Lenka (UEB-Q)_{ORCID, RID} Hrdlička, Jakub (UEB-Q)_ORCID Smýkalová, I. (CZ) Cvečková, M. (CZ) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Griga, M. (CZ) Doležal, Karel (UEB-Q)_{RID, ORCID}
Source Title	Plant Growth Regulation. - : Springer - ISSN 0167-6903 Roč. 77, č. 2 (2015), s. 125-132
Number of pages	8 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Cytokinins ; Pea ; Quantification
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000362688300003
DOI	10.1007/s10725-015-0044-z
Annotation	Forty-seven endogenous cytokinin metabolites were determined in samples of long-term in vitro multiple shoot cultures of pea (Pisum sativum L.). Significant differences were found in the levels of all cytokinin groups except cis-zeatin and some of its derivatives. Old cultures (30 or 10 years old) contained larger total and active cytokinin pools than freshly prepared cultures. Long-term shoot cultures maintained on cytokinin-supplemented media initially exhibit gradually increasing concentrations of endogenous cytokinins that subsequently stabilise at a high level, which can make it more difficult to induce rooting later on. This is consistent with previous findings concerning the growth and development of plantlets derived from long-term cultures and the generally accepted role of cytokinins during root development. The results presented herein demonstrate the utility of long-term pea cultures as model systems for studying the influence of long-term cultivation on genetic stability, and suggest ways of circumventing the rooting problems associated with such cultures.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2016

Number of the records: 1