Challenges of flow-cytometric estimation of nuclear genome size in orchids, a plant group with both whole-genome and progressively partial endoreplication

Trávníček, Pavel; Ponert, J.; Urfus, Tomáš; Jersáková, Jana; Vrána, Jan; Hřibová, Eva; Doležel, Jaroslav; Suda, Jan

doi:https://dx.doi.org/10.1002/cyto.a.22681

Number of the records: 1

Challenges of flow-cytometric estimation of nuclear genome size in orchids, a plant group with both whole-genome and progressively partial endoreplication

1.

SYSNO ASEP	0449202
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Challenges of flow-cytometric estimation of nuclear genome size in orchids, a plant group with both whole-genome and progressively partial endoreplication
Author(s)	Trávníček, Pavel (BU-J)_{RID, ORCID} Ponert, J. (CZ) Urfus, Tomáš (BU-J)_RID Jersáková, Jana (UEK-B)_{RID, SAI, ORCID} Vrána, Jan (UEB-Q)_{RID, ORCID} Hřibová, Eva (UEB-Q)_{RID, ORCID} Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Suda, Jan (BU-J)_RID
Number of authors	8
Source Title	Cytometry. Part A. - : Wiley - ISSN 1552-4922 Roč. 87, č. 10 (2015), s. 958-966
Number of pages	9 s.
Language	eng - English
Country	US - United States
Keywords	flow cytometry ; genome size ; hyporeduplication
Subject RIV	EF - Botanics
Subject RIV - cooperation	Institute of Experimental Botany - Botanics Global Change Research Institute - Ecology, Behaviour
R&D Projects	GAP506/12/1320 GA ČR - Czech Science Foundation (CSF)
Institutional support	BU-J - RVO:67985939 ; RVO:67179843 - RVO:67179843 ; UEB-Q - RVO:61389030
UT WOS	000363042900011
EID SCOPUS	84942369482
DOI	10.1002/cyto.a.22681
Annotation	We analyzed six different tissue types in 48 orchid species belonging to all recognized subfamilies. Although traditionally used leaves may provide incorrect C-values, particularly in species with progressively partial endoreplication, young ovaries and pollinaria consistently yield 2C and 1C peaks of their G1-phase nuclei, respectively, and are, therefore, the most suitable parts for genome size studies in orchids. We also provide new DNA C-values for 22 orchid genera and 42 species.
Workplace	Institute of Botany
Contact	Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8
Year of Publishing	2016

Number of the records: 1