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Forward genetic screen for auxin-deficient mutants by cytokinin
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SYSNO ASEP 0447201 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Forward genetic screen for auxin-deficient mutants by cytokinin Author(s) Wu, L. (CN)
Luo, P. (CN)
Di, D.W. (CN)
Wang, L. (CN)
Wang, M. (CN)
Lu, C.K. (CN)
Wei, S.D. (CN)
Zhang, L. (CN)
Zhang, T.Z. (CN)
Amakorová, Petra (UEB-Q) ORCID
Strnad, Miroslav (UEB-Q) RID, ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Guo, G.Q. (CN)Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 5, JUL 6 (2015)Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords ETHYLENE-INSENSITIVE MUTANTS ; YUCCA FLAVIN MONOOXYGENASES ; ARABIDOPSIS-THALIANA Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000357376500004 DOI 10.1038/srep11923 Annotation Identification of mutants with impairments in auxin biosynthesis and dynamics by forward genetic screening is hindered by the complexity, redundancy and necessity of the pathways involved. Furthermore, although a few auxin-deficient mutants have been recently identified by screening for altered responses to shade, ethylene, N-1-naphthylphthalamic acid (NPA) or cytokinin (CK), there is still a lack of robust markers for systematically isolating such mutants. We hypothesized that a potentially suitable phenotypic marker is root curling induced by CK, as observed in the auxin biosynthesis mutant CK-induced root curling 1 / tryptophan aminotransferase of Arabidopsis 1 (ckrc1/taa1). Phenotypic observations, genetic analyses and biochemical complementation tests of Arabidopsis seedlings displaying the trait in large-scale genetic screens showed that it can facilitate isolation of mutants with perturbations in auxin biosynthesis, transport and signaling. However, unlike transport/signaling mutants, the curled (or wavy) root phenotypes of auxin-deficient mutants were significantly induced by CKs and could be rescued by exogenous auxins. Mutants allelic to several known auxin biosynthesis mutants were re-isolated, but several new classes of auxin-deficient mutants were also isolated. The findings show that CK-induced root curling provides an effective marker for discovering genes involved in auxin biosynthesis or homeostasis. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
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