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Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides
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SYSNO ASEP 0447026 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides Author(s) Akpinar, B.A. (TR)
Yuce, M. (TR)
Lucas, S. (TR)
Vrána, Jan (UEB-Q) RID, ORCID
Burešová, Veronika (UEB-Q) RID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Budak, H. (TR)Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 5, JUN 18 (2015)Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords TURGIDUM VAR. DICOCCOIDES ; MARKER DEVELOPMENT ; GENOME SEQUENCE Subject RIV EB - Genetics ; Molecular Biology R&D Projects GBP501/12/G090 GA ČR - Czech Science Foundation (CSF) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000356524700001 DOI 10.1038/srep10763 Annotation Wild emmer wheat, Triticum turgidum ssp. dicoccoides is the wild relative of Triticum turgidum, the progenitor of durum and bread wheat, and maintains a rich allelic diversity among its wild populations. The lack of adequate genetic and genomic resources, however, restricts its exploitation in wheat improvement. Here, we report next-generation sequencing of the flow-sorted chromosome 5B of T. dicoccoides to shed light into its genome structure, function and organization by exploring the repetitive elements, protein-encoding genes and putative microRNA and tRNA coding sequences. Comparative analyses with its counterparts in modern and wild wheats suggest clues into the B-genome evolution. Syntenic relationships of chromosome 5B with the model grasses can facilitate further efforts for fine-mapping of traits of interest. Mapping of 5B sequences onto the root transcriptomes of two additional T. dicoccoides genotypes, with contrasting drought tolerances, revealed several thousands of single nucleotide polymorphisms, of which 584 shared polymorphisms on 228 transcripts were specific to the drought-tolerant genotype. To our knowledge, this study presents the largest genomics resource currently available for T. dicoccoides, which, we believe, will encourage the exploitation of its genetic and genomic potential for wheat improvement to meet the increasing demand to feed the world. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
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