Number of the records: 1
Ammonium regulates embryogenic potential in Cucurbita pepo through pH-mediated changes in endogenous auxin and abscisic acid
- 1.
SYSNO ASEP 0446932 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ammonium regulates embryogenic potential in Cucurbita pepo through pH-mediated changes in endogenous auxin and abscisic acid Author(s) Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Turečková, Veronika (UEB-Q) RID, ORCID
Paulisić, S. (HR)
Rolčík, Jakub (UEB-Q)
Strnad, Miroslav (UEB-Q) RID, ORCID
Mihaljević, S. (HR)Source Title Plant Cell, Tissue and Organ Culture. - : Springer - ISSN 0167-6857
Roč. 122, č. 1 (2015), s. 89-100Number of pages 12 s. Language eng - English Country NL - Netherlands Keywords Abscisic acid ; Ammonium ; Indole-3-acetic acid Subject RIV EB - Genetics ; Molecular Biology Institutional support UEB-Q - RVO:61389030 UT WOS 000355921800009 DOI 10.1007/s11240-015-0752-0 Annotation When supplied as the sole nitrogen source, ammonium can induce somatic embryogenesis in some plant species, including pumpkin. To get an insight into the involvement of phytohormones in this adaptive developmental response to nitrogen availability, the status of endogenous abscisic acid (ABA), indole-3-acetic acid (IAA), and their conjugates was analyzed in an ammonium-induced embryogenic pumpkin culture. Results were compared with endogenous hormone status in an embryogenic culture induced by exogenously supplied 2,4-dichlorophenoxyacetic acid (2,4-D). In the NH4 (+)-induced somatic embryogenic culture, the maximum concentration of ABA was measured after 21 days of cultivation, while in the 2,4-D-induced culture ABA peaked after 7 days of cultivation, which suggested a possible activation of different ABA-regulated stress responses. The NH4 (+)-induced embryogenic culture was also characterized by increased accumulation of endogenous IAA to the level similar or higher than the one induced by 2,4-D. A considerable decrease (threefold) in endogenous IAA content was observed after buffering the NH4 (+)-medium with MES or after nitrate re-supply. In contrast, buffering the NH4 (+)-medium increased endogenous ABA by five times. Significant ammonium- and pH-related changes were also detected in ascorbate and glutathione redox status, and discussed regarding the corresponding changes in cell size and mitotic activity in the cultures. Furthermore, a negative correlation between free IAA content and the activity of ascorbate peroxidase (EC 1.11.1.11) and auxin-sensitive peroxidase CpAPRX (EC 1.11.1.7) was observed. The results strongly suggest a mediating role of external pH in the regulation of endogenous phytohormones and the ascorbate-glutathione cycle during ammonium-induced acquisition of embryogenic competence in pumpkin. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
Number of the records: 1