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Tomato (Solanum lycopersicum L.) SlIPT3 and SlIPT4 isopentenyltransferases mediate salt stress response in tomato
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SYSNO ASEP 0446710 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Tomato (Solanum lycopersicum L.) SlIPT3 and SlIPT4 isopentenyltransferases mediate salt stress response in tomato Author(s) Žižková, Eva (UEB-Q) ORCID
Dobrev, Petre (UEB-Q) RID, ORCID
Muhovski, Y. (BE)
Hošek, Petr (UEB-Q) ORCID
Hoyerová, Klára (UEB-Q) RID, ORCID
Haisel, Daniel (UEB-Q) RID, ORCID
Procházková, Dagmar (UEB-Q) RID
Lutts, S. (BE)
Motyka, Václav (UEB-Q) RID, ORCID
Hichri, I. (BE)Source Title BMC Plant Biology. - : BioMed Central - ISSN 1471-2229
Roč. 15, MAR 12 (2015)Number of pages 20 s. Language eng - English Country GB - United Kingdom Keywords Cytokinin ; Isopentenyltransferase ; Salt stress Subject RIV EF - Botanics R&D Projects GAP506/11/0774 GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000353267000001 DOI 10.1186/s12870-015-0415-7 Annotation Background: Cytokinins (CKs) are involved in response to various environmental cues, including salinity. It has been previously reported that enhancing CK contents improved salt stress tolerance in tomato. However, the underlying mechanisms of CK metabolism and signaling under salt stress conditions remain to be deciphered. Results: Two tomato isopentenyltransferases, SlIPT3 and SlIPT4, were characterized in tomato and Arabidopsis. Both proteins displayed isopentenyltransferase (IPT) activity in vitro, while their encoding genes exhibited different spatio-temporal expression patterns during tomato plant development. SlIPT3 and SlIPT4 were affected by the endogenous CK status, tightly connected with CKs feedback regulation, as revealed by hormonal treatements. In response to salt stress, SlIPT3 and SlIPT4 were strongly repressed in tomato roots, and differently affected in young and old leaves. SlIPT3 overexpression in tomato resulted in high accumulation of different CK metabolites, following modifications of CK biosynthesis-, signaling- and degradation-gene expression. In addition, 35S::SlIPT3 tomato plants displayed improved tolerance to salinity consecutive to photosynthetic pigments and K+/Na+ ratio retention. Involvement of SlIPT3 and SlIPT4 in salt stress response was also observed in Arabidopsis ipt3 knock-out complemented plants, through maintenance of CK homeostasis. Conclusions: SlIPT3 and SlIPT4 are functional IPTs encoded by differently expressed genes, distinctively taking part in the salinity response. The substantial participation of SlIPT3 in CK metabolism during salt stress has been determined in 35S::SlIPT3 tomato transformants, where enhancement of CKs accumulation significantly improved plant tolerance to salinity, underlining the importance of this phytohormone in stress response. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
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