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PHABULOSA Controls the Quiescent Center-Independent Root Meristem Activities in Arabidopsis thaliana
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SYSNO ASEP 0446678 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title PHABULOSA Controls the Quiescent Center-Independent Root Meristem Activities in Arabidopsis thaliana Author(s) Sebastian, J. (US)
Ryu, K.H. (KR)
Zhou, J. (US)
Tarkowská, Danuše (UEB-Q) RID, ORCID
Tarkowski, P. (CZ)
Cho, Y.H. (KR)
Yoo, S.D. (KR)
Kim, E.S. (KR)
Lee, J.Y. (US)Source Title PLoS Genetics - ISSN 1553-7390
Roč. 11, č. 3 (2015)Number of pages 27 s. Language eng - English Country US - United States Keywords STEM-CELL NICHE ; POSTTRANSCRIPTIONAL REGULATION ; GENE-EXPRESSION Subject RIV EB - Genetics ; Molecular Biology Institutional support UEB-Q - RVO:61389030 UT WOS 000352197100003 DOI 10.1371/journal.pgen.1004973 Annotation Plant growth depends on stem cell niches in meristems. In the root apical meristem, the quiescent center (QC) cells form a niche together with the surrounding stem cells. Stem cells produce daughter cells that are displaced into a transit-amplifying (TA) domain of the root meristem. TA cells divide several times to provide cells for growth. SHORTROOT (SHR) and SCARECROW (SCR) are key regulators of the stem cell niche. Cytokinin controls TA cell activities in a dose-dependent manner. Although the regulatory programs in each compartment of the root meristem have been identified, it is still unclear how they coordinate one another. Here, we investigate how PHABULOSA (PHB), under the post-transcriptional control of SHR and SCR, regulates TA cell activities. The root meristem and growth defects in shr or scr mutants were significantly recovered in the shr phb or scr phb double mutant, respectively. This rescue in root growth occurs in the absence of a QC. Conversely, when the modified PHB, which is highly resistant to microRNA, was expressed throughout the stele of the wild-type root meristem, root growth became very similar to that observed in the shr; however, the identity of the QC was unaffected. Interestingly, a moderate increase in PHB resulted in a root meristem phenotype similar to that observed following the application of high levels of cytokinin. Our protoplast assay and transgenic approach using ARR10 suggest that the depletion of TA cells by high PHB in the stele occurs via the repression of B-ARR activities. This regulatory mechanism seems to help to maintain the cytokinin homeostasis in the meristem. Taken together, our study suggests that PHB can dynamically regulate TA cell activities in a QC-independent manner, and that the SHR-PHB pathway enables a robust root growth system by coordinating the stem cell niche and TA domain. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
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