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pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes
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SYSNO ASEP 0446475 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes Author(s) Hanneken, M. (DE)
Šlais, Karel (UIACH-O) RID, ORCID
König, S. (DE)Number of authors 3 Source Title Data in Brief. - : Elsevier - ISSN 2352-3409
Roč. 3, JUN (2015), s. 221-228Number of pages 8 s. Publication form Online - E Language eng - English Country DE - Germany Keywords 2D-PAGE ; CoFGE ; Gel electrophoresis Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Method of publishing Open access Institutional support UIACH-O - RVO:68081715 UT WOS 000453156300042 EID SCOPUS 84928104880 DOI 10.1016/j.euprot.2015.03.003 Annotation Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by isoelectric focussing are presented. The latter were used to correct for variation in the first electrophoretic dimension and further improve protein coordinate assignment in 2D-gel electrophoresis. Data tables are supplied to demonstrate pI-value calibration and the effect on the assignment of protein spot coordinates. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2016 Electronic address http://www.sciencedirect.com/science/article/pii/S2352340915000414
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