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Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants grown in vitro
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SYSNO ASEP 0446124 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants grown in vitro Author(s) Trifunovic, M. (RS)
Motyka, Václav (UEB-Q) RID, ORCID
Cingel, A. (RS)
Subotic, A. (RS)
Jevremovic, S. (RS)
Petric, M. (RS)
Holík, Josef (UEB-Q) RID, ORCID
Malbeck, Jiří (UEB-Q) RID, ORCID
Dobrev, Petre (UEB-Q) RID, ORCID
Dragicevic, I. (RS)Source Title Plant Cell, Tissue and Organ Culture. - : Springer - ISSN 0167-6857
Roč. 120, č. 2 (2015), s. 767-777Number of pages 11 s. Language eng - English Country NL - Netherlands Keywords Centaurium erythraea Rafn. ; Cytokinin ; Cytokinin oxidase/dehydrogenase Subject RIV EF - Botanics R&D Projects GAP506/11/0774 GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000348807200031 DOI 10.1007/s11240-014-0646-6 Annotation The plant hormones cytokinins (CKs) regulate a number of physiological processes. Their homeostasis is controlled by the rate of de novo synthesis and the rate of catabolism. The aim of this work was to analyze the content of total as well as individual groups of endogenous CKs in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants grown in vitro. Transgenic CKX plants represent a suitable model system for studying physiological and morphological processes controlled by CKs. In this work we clearly demonstrate a significant effect of AtCKX transgenes on CK metabolism in transgenic centaury plants. However, shoots and roots of only one AtCKX1 line and three AtCKX2 lines with a significant reduction of bioactive CKs were obtained. We also show that changes in the CKs metabolism considerably affected endogenous indole-3-acetic acid (IAA) levels in plant tissues. All analyzed transgenic AtCKX centaury lines exhibited decreased amount of endogenous IAA in shoots as well as in roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation in the shoots and roots of all analyzed AtCKX centaury transformants. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
Number of the records: 1