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Various Extraction Methods for Obtaining Stilbenes from Grape Cane of Vitis vinifera L
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SYSNO ASEP 0443166 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Various Extraction Methods for Obtaining Stilbenes from Grape Cane of Vitis vinifera L Author(s) Soural, I. (CZ)
Vrchotová, Naděžda (UEK-B) RID, SAI, ORCID
Tříska, Jan (UEK-B) RID, ORCID, SAI
Balík, J. (CZ)
Horník, Štěpán (UCHP-M) ORCID, RID, SAI
Cuřínová, Petra (UCHP-M) RID, SAI, ORCID
Sýkora, Jan (UCHP-M) RID, ORCID, SAISource Title Molecules. - : MDPI
Roč. 20, č. 4 (2015), s. 6093-6112Number of pages 20 s. Language eng - English Country CH - Switzerland Keywords Vitis vinifera L ; grape cane ; stilbenes ; accelerated solvent extraction (ASE) ; microwave-assisted extraction (MAE) ; LC-MS Subject RIV CB - Analytical Chemistry, Separation R&D Projects LD14038 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support RVO:67179843 - RVO:67179843 ; UCHP-M - RVO:67985858 UT WOS 000354480700052 EID SCOPUS 84928663035 DOI 10.3390/molecules20046093 Annotation Grape cane, leaves and grape marc are waste products from viticulture, which can be used to obtain secondary stilbene derivatives with high antioxidant value. The presented work compares several extraction methods: maceration at laboratory temperature, extraction at elevated temperature, fluidized-bed extraction, Soxhlet extraction, microwave-assisted extraction, and accelerated solvent extraction. To obtain trans-resveratrol, trans-ε-viniferin and r2-viniferin from grape cane of the V. vinifera variety Cabernet Moravia, various conditions were studied: different solvents, using powdered versus cut cane material, different extraction times, and one-step or multiple extractions. The largest concentrations found were 6030 ± 680 µg/g dry weight (d.w.) for trans-resveratrol, 2260 ± 90 µg/g d.w. for trans-ε-viniferin, and 510 ± 40 µg/g d.w. for r2-viniferin. The highest amounts of stilbenes (8500 ± 1100 µg/g d.w.) were obtained using accelerated solvent extraction in methanol. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2016
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