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Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia

SYSNO ASEP	0436625
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia
Author(s)	Králová, Tereza (BFU-R) Čegan, Radim (BFU-R)_{RID, ORCID} Kubát, Zdeněk (BFU-R)_{RID, ORCID} Vrána, Jan (UEB-Q)_{RID, ORCID} Vyskot, Boris (BFU-R)_{RID, ORCID} Vogel, Ivan (BFU-R) Kejnovský, Eduard (BFU-R)_{RID, ORCID} Hobza, Roman (BFU-R)_{RID, ORCID}
Number of authors	8
Source Title	Cytogenetic and Genome Research. - : Karger - ISSN 1424-8581 Roč. 143, 1-3 (2014), s. 87-95
Number of pages	9 s.
Publication form	Print - P
Language	eng - English
Country	CH - Switzerland
Keywords	Microdissection ; Sex chromosomes ; Silene latifolia (white campion)
Subject RIV	BO - Biophysics
Subject RIV - cooperation	Institute of Experimental Botany - Botanics
R&D Projects	LM2010005 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GAP501/10/0102 GA ČR - Czech Science Foundation (CSF)
	GAP501/12/2220 GA ČR - Czech Science Foundation (CSF)
	GAP305/10/0930 GA ČR - Czech Science Foundation (CSF)
	GA522/09/0083 GA ČR - Czech Science Foundation (CSF)
	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BFU-R - RVO:68081707
UT WOS	000342614100009
DOI	10.1159/000362142
Annotation	Silene latifolia is a dioecious plant species with chromosomal sex determination. Although the evolution of sex chromosomes in S. latifolia has been the subject of numerous studies, a global view of X chromosome structure in this species is still missing. Here, we combine X chromosome microdissection and BAC library screening to isolate new X chromosome-linked sequences. Out of 8 identified BAC clones, only BAC 86M14 showed an X-preferential signal after FISH experiments. Further analysis revealed the existence of the Athila retroelement which is enriched in the X chromosome and nearly absent in the Y chromosome. Based on previous data, the Athila retroelement belongs to the CL3 group of most repetitive sequences in the S. latifolia genome. Structural, transcriptomics and phylogenetic analyses revealed that Athila CL3 represents an old clade in the Athila lineage. We propose a mechanism responsible for Athila CL3 distribution in the S. latifolia genome. (C) 2014 S. Karger AG, Basel
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2015

Number of the records: 1