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Bordetella Adenylate Cyclase Toxin Differentially Modulates Toll-Like Receptor-Stimulated Activation, Migration and T Cell Stimulatory Capacity of Dendritic Cells

  1. 1.
    SYSNO ASEP0435908
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleBordetella Adenylate Cyclase Toxin Differentially Modulates Toll-Like Receptor-Stimulated Activation, Migration and T Cell Stimulatory Capacity of Dendritic Cells
    Author(s) Adkins, Irena (MBU-M)
    Kamanová, Jana (MBU-M) ORCID, RID
    Kocourková, A. (CZ)
    Švédová, Martina (MBU-M)
    Tomala, Jakub (MBU-M) RID, ORCID
    Janová, H. (CZ)
    Mašín, Jiří (MBU-M) RID, ORCID
    Chládková, Barbara (MBU-M) RID
    Bumba, Ladislav (MBU-M) RID, ORCID
    Kovář, Marek (MBU-M) RID, ORCID
    Ross, P. J. (IE)
    Tučková, Ludmila (MBU-M) RID
    Spíšek, R. (CZ)
    Mills, K. H. G. (IE)
    Šebo, Peter (MBU-M) RID, ORCID
    Source TitlePLoS ONE. - : Public Library of Science - ISSN 1932-6203
    Roč. 9, č. 8 (2014)
    Number of pages13 s.
    Languageeng - English
    CountryUS - United States
    KeywordsRESPIRATORY-INFECTION ; INTERLEUKIN-10 PRODUCTION ; PROTECTIVE IMMUNITY
    Subject RIVEE - Microbiology, Virology
    R&D ProjectsGA310/08/0447 GA ČR - Czech Science Foundation (CSF)
    GP310/09/P582 GA ČR - Czech Science Foundation (CSF)
    GAP301/11/0325 GA ČR - Czech Science Foundation (CSF)
    1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportMBU-M - RVO:61388971
    UT WOS000339819800123
    DOI10.1371/journal.pone.0104064
    AnnotationAdenylate cyclase toxin (CyaA) is a key virulence factor of the whooping cough agent Bordetella pertussis. The toxin targets CD11b-expressing phagocytes and delivers into their cytosol an adenylyl cyclase (AC) enzyme that subverts cellular signaling by increasing cAMP levels. In the present study, we analyzed the modulatory effects of CyaA on adhesive, migratory and antigen presenting properties of Toll-like receptor (TLR)-activated murine and human dendritic cells (DCs). cAMP signaling of CyaA enhanced TLR-induced dissolution of cell adhesive contacts and migration of DCs towards the lymph node-homing chemokines CCL19 and CCL21 in vitro. Moreover, we examined in detail the capacity of toxin-treated DCs to induce CD4(+) and CD8(+) T cell responses. Exposure to CyaA decreased the capacity of LPS-stimulated DCs to present soluble protein antigen to CD4(+) T cells independently of modulation of co-stimulatory molecules and cytokine production, and enhanced their capacity to promote CD4(+) CD25(+) Foxp3(+) T regulatory cells in vitro. In addition, CyaA decreased the capacity of LPS-stimulated DCs to induce CD8(+) T cell proliferation and limited the induction of IFN-gamma producing CD8(+) T cells while enhancing IL-10 and IL-17-production. These results indicate that through activation of cAMP signaling, the CyaA may be mobilizing DCs impaired in T cell stimulatory capacity and arrival of such DCs into draining lymph nodes may than contribute to delay and subversion of host immune responses during B. pertussis infection
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2015
Number of the records: 1  

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