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UHPLC-MS/MS based target profiling of stress-induced phytohormones

SYSNO ASEP	0433242
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	UHPLC-MS/MS based target profiling of stress-induced phytohormones
Author(s)	Floková, Kristýna (UEB-Q)_{ORCID, RID} Tarkowská, Danuše (UEB-Q)_{RID, ORCID} Miersch, Otto (UEB-Q)_{RID, SAI} Strnad, Miroslav (UEB-Q)_{RID, ORCID} Wasternack, Claus (UEB-Q)_ORCID Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI}
Source Title	Phytochemistry. - : Elsevier - ISSN 0031-9422 Roč. 105, SEP 2014 (2014), s. 147-157
Number of pages	11 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Stress-induced phytohormones ; Jasmonates ; Abscisic acid
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	GA14-34792S GA ČR - Czech Science Foundation (CSF)
	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000340986900019
DOI	10.1016/j.phytochem.2014.05.015
Annotation	Stress-induced changes in phytohormone metabolite profiles have rapid effects on plant metabolic activity and growth. The jasmonates (JAs) are a group of fatty acid-derived stress response regulators with roles in numerous developmental processes. To elucidate their dual regulatory effects, which overlap with those of other important defence-signalling plant hormones such as salicylic acid (SA), abscisic acid (ABA) and indole-3-acetic acid (IAA), we have developed a highly efficient single-step clean-up procedure for their enrichment from complex plant matrices that enables their sensitive quantitative analysis using hyphenated mass spectrometry technique. The rapid extraction of minute quantities of plant material (less than 20 mg fresh weight. FW) into cold 10% methanol followed by one-step reversed-phase polymer-based solid phase extraction significantly reduced matrix effects and increased the recovery of labile JA analytes. This extraction and purification protocol was paired with a highly sensitive and validated ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method and used to simultaneously profile sixteen stress-induced phytohormones in minute plant material samples, including endogenous JA, several of its biosynthetic precursors and derivatives, as well as SA, ABA and IAA.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2015

Number of the records: 1