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The Pseudomonas type III effector HopQ1 activates cytokinin signaling and interferes with plant innate immunity
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SYSNO ASEP 0431074 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Pseudomonas type III effector HopQ1 activates cytokinin signaling and interferes with plant innate immunity Author(s) Hann, D.R. (CH)
Dominguez-Ferreras, A. (DE)
Motyka, Václav (UEB-Q) RID, ORCID
Dobrev, Petre (UEB-Q) RID, ORCID
Schornack, S. (GB)
Jehle, A. (DE)
Felix, G. (DE)
Chinchilla, D. (DE)
Rathjen, J.P. (AT)
Boller, T. (DE)Source Title New Phytologist - ISSN 0028-646X
Roč. 201, č. 2 (2014), s. 585-598Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords cytokinin ; flagellin ; FLS2 Subject RIV GF - Plant Pathology, Vermin, Weed, Plant Protection R&D Projects GA13-26798S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000328511700022 DOI 10.1111/nph.12544 Annotation We characterized the molecular function of the Pseudomonas syringae pv. tomato DC3000 (Pto) effector HopQ1.In silico studies suggest that HopQ1 might possess nucleoside hydrolase activity based on the presence of a characteristic aspartate motif. Transgenic Arabidopsis lines expressing HopQ1 or HopQ1 aspartate mutant variants were characterized with respect to flagellin triggered immunity, phenotype and changes in phytohormone content by high-performance liquid chromatography-MS (HPLC-MS). We found that HopQ1, but not its aspartate mutants, suppressed all tested immunity marker assays. Suppression of immunity was the result of a lack of the flagellin receptor FLS2, whose gene expression was abolished by HopQ1 in a promoter-dependent manner. Furthermore, HopQ1 induced cytokinin signaling in Arabidopsis and the elevation in cytokinin signaling appears to be responsible for the attenuation of FLS2 expression. We conclude that HopQ1 can activate cytokinin signaling and that moderate activation of cytokinin signaling leads to suppression of FLS2 accumulation and thus defense signaling. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2015
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