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Molecular mapping of stripe rust resistance gene Yr51 in chromosome 4AL of wheat

SYSNO ASEP	0429990
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Molecular mapping of stripe rust resistance gene Yr51 in chromosome 4AL of wheat
Author(s)	Randhawa, M. (AU) Bansal, U. (AU) Valárik, Miroslav (UEB-Q)_{RID, ORCID} Klocová, Barbora (UEB-Q)_RID Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Bariana, H. (AU)
Source Title	Theoretical and Applied Genetics. - : Springer - ISSN 0040-5752 Roč. 127, č. 2 (2014), s. 317-324
Number of pages	8 s.
Language	eng - English
Country	DE - Germany
Keywords	TRITICUM-AESTIVUM L. ; DIVERSITY ARRAYS ; MAP
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GAP501/10/1740 GA ČR - Czech Science Foundation (CSF)
	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000330723900006
DOI	10.1007/s00122-013-2220-8
Annotation	This manuscript describes the chromosomal location of a new source of stripe rust resistance in wheat. DNA markers closely linked with the resistance locus were identified and validated. A wheat landrace, AUS27858, from the Watkins collection showed high levels of resistance against Australian pathotypes of Puccinia striiformis f. sp. tritici. It was reported to carry two genes for stripe rust resistance, tentatively named YrAW1 and YrAW2. One hundred seeds of an F3 line (HSB#5515; YrAW1yrAW1) that showed monogenic segregation for stripe rust response were sown and harvested individually to generate monogenically segregating population (MSP) #5515. Stripe rust response variation in MSP#5515 conformed to segregation at a single locus. Bulked segregant analysis using high-throughput DArT markers placed YrAW1 in chromosome 4AL. MSP#5515 was advanced to F6 and phenotyped for detailed mapping. Novel wheat genomic resources including chromosome-specific sequence and genome zipper were employed to develop markers specific for the long arm of chromosome 4A. These markers were used for further saturation of the YrAW1 carrying region. YrAW1 was delimited by 3.7 cM between markers owm45F3R3 and sun104. Since there was no other stripe rust resistance gene located in chromosome 4AL, YrAW1 was formally named Yr51. Reference stock for Yr51 was lodged at the Australian Winter Cereal Collection, Tamworth, Australia and it was accessioned as AUS91456. Marker sun104 was genotyped on a set of Australian and Indian wheat cultivars and was shown to lack the resistance-linked sun104-225 bp allele. Marker sun104 is currently being used for marker-assisted backcrossing of Yr51 in Australian and Indian wheat backgrounds.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2015

Number of the records: 1