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Phosphoenolpyruvate carboxylase, NADP-malic enzyme, and pyruvate, phosphate dikinase are involved in the acclimation of Nicotiana tabacum L. to drought stress
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SYSNO ASEP 0429982 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Phosphoenolpyruvate carboxylase, NADP-malic enzyme, and pyruvate, phosphate dikinase are involved in the acclimation of Nicotiana tabacum L. to drought stress Author(s) Doubnerová-Hýsková, V. (CZ)
Miedzińska, L. (CZ)
Dobrá, Jana (UEB-Q)
Vaňková, Radomíra (UEB-Q) RID, ORCID
Ryšlavá, H. (CZ)Source Title Journal of Plant Physiology. - : Elsevier - ISSN 0176-1617
Roč. 171, č. 5 (2014), s. 19-25Number of pages 7 s. Language eng - English Country DE - Germany Keywords Drought ; NADP-malic enzyme ; Nicotiana tabacum L. Subject RIV EI - Biotechnology ; Bionics R&D Projects 1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000331925900004 DOI 10.1016/j.jplph.2013.10.017 Annotation Drought stress is one of the most frequent forms of abiotic stresses, which occurs under condition of limited water availability. In this work, the possible participation of phosphoenolpyruvate carboxylase (EC 4.1.1.31; PEPC), NADP-malic enzyme (EC 1.1.1.40; NADP-ME), and pyruvate, phosphate dikinase (EC 2.7.9.1; PPDK) in response to drought of tobacco plants (Nicotiana tabacum L, cv. W38) was investigated. Enzyme specific activities in tobacco leaves of drought stressed plants were significantly increased after 11 days of stress, PEPC 2.3-fold, NADP-ME 3.9-fold, and PPDK 2.7-fold compared to control plants. The regulation of PEPC and NADP-ME activities were studied on transcriptional level by the quantitative RT PCR and on translational level - immunochemically. The amount of NADP-ME protein and transcription of mRNA for chloroplastic NADP-ME isoform were increased indicating their enhanced synthesis de novo. On the other hand, mRNA for cytosolic isoform of NADP-ME was decreased. The changes in PEPC protein and PEPC mRNA were not substantial. Therefore regulation of PEPC activity by phosphorylation was evaluated and found to be involved in the stress response. During recovery, activities of the tested enzymes returned close to their basal levels. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2015
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