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Assessment of somaclonal variation in somatic embryo-derived plants of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson] using inter simple sequence repeat analysis and flow cytometry
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SYSNO ASEP 0429935 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Assessment of somaclonal variation in somatic embryo-derived plants of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson] using inter simple sequence repeat analysis and flow cytometry Author(s) Viehmannová, I. (CZ)
Bortlová, Z. (CZ)
Vítámvás, J. (CZ)
Čepková, P. (CZ)
Eliášová, Kateřina (UEB-Q) RID, ORCID
Svobodová, E. (CZ)
Trávníčková, M. (CZ)Source Title Electronic Journal of Biotechnology - ISSN 0717-3458
Roč. 17, č. 2 (2014)Number of pages 5 s. Language eng - English Country CL - Chile Keywords DNA polymorphism ; Molecular markers ; Ploidy level Subject RIV EB - Genetics ; Molecular Biology Institutional support UEB-Q - RVO:61389030 UT WOS 000334441800007 DOI 10.1016/j.ejbt.2013.12.011 Annotation Background: Yacon (Smallanthus sonchifolius) is a root crop native to the Andean region. Low sexual reproductive capacity is a major constraint facing the genetic breeding of this crop. Biotechnological techniques offer alternative ways to widen genetic variability. We investigated somaclonal variation in regenerants of yacon derived from in vitro somatic embryogenesis using simple sequence repeat (ISSR) analysis and flow cytometry. Results: Twenty tested ISSR primers provided a total of 7848 bands in 60 in vitro regenerants and control plant. The number of bands for each primer varied from 3 to 10, and an average of 6.95 bands was obtained per ISSR primer. Eight primers were polymorphic and generated 10 polymorphic bands with 7.19% mean polymorphism. ISSR analysis revealed genetic variability in 6 plants under study. These regenerants had Jaccard's distances 0.104, 0.020, 0.040, 0.106, 0.163 and 0.040. Flow cytometric analysis did not reveal changes of relative nuclear DNA content in regenerants suggesting that the plants obtained via somatic embryogenesis had maintained stable octoploid levels. Conclusions: Our findings show that indirect somatic embryogenesis could be used in yacon improvement to widen genetic variability, especially when low sexual reproductive capacity hinders classical ways of breeding. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2015
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