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Flow cytometric chromosome sorting from diploid progenitors of bread wheat, T. urartu, Ae. speltoides and Ae. tauschii

SYSNO ASEP	0429328
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Flow cytometric chromosome sorting from diploid progenitors of bread wheat, T. urartu, Ae. speltoides and Ae. tauschii
Author(s)	Molnár, I. (HU) Kubaláková, Marie (UEB-Q)_RID Šimková, Hana (UEB-Q)_{RID, ORCID} Farkas, A. (HU) Cseh, A. (HU) Megyeri, M. (HU) Vrána, Jan (UEB-Q)_{RID, ORCID} Molnár-Láng, M. (HU) Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Source Title	Theoretical and Applied Genetics. - : Springer - ISSN 0040-5752 Roč. 127, č. 5 (2014), s. 1091-1104
Number of pages	14 s.
Language	eng - English
Country	DE - Germany
Keywords	SYNTHETIC HEXAPLOID WHEAT ; AEGILOPS-TRITICUM GROUP ; GENETIC-LINKAGE MAP
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GBP501/12/G090 GA ČR - Czech Science Foundation (CSF)
	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000336754700007
DOI	10.1007/s00122-014-2282-2
Annotation	The three subgenomes of hexaploid bread wheat originated from Triticum urartu (A(u)A(u)), from a species similar to Aegilops speltoides (SS) (progenitor of the B genome), and from Ae. tauschii (DD). Earlier studies indicated the potential of chromosome genomics to assist gene transfer from wild relatives of wheat and discover novel genes for wheat improvement. This study evaluates the potential of flow cytometric chromosome sorting in the diploid progenitors of bread wheat. Flow karyotypes obtained by analysing DAPI-stained chromosomes were characterized and the contents of the chromosome peaks were determined. FISH analysis with repetitive DNA probes proved that chromosomes 5A(u), 5S and 5D could be sorted with purities of 78-90 %, while the remaining chromosomes could be sorted in groups of three. Twenty-five conserved orthologous set (COS) markers covering wheat homoeologous chromosome groups 1-7 were used for PCR with DNA amplified from flow-sorted chromosomes and genomic DNA. These assays validated the cytomolecular results as follows: peak I on flow karyotypes contained chromosome groups 1, 4 and 6, peak II represented homoeologous group 5, while peak III consisted of groups 2, 3 and 7. The isolation of individual chromosomes of wild progenitors provides an attractive opportunity to investigate the structure and evolution of the polyploid genome and to deliver tools for wheat improvement.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2015

Number of the records: 1