Number of the records: 1

Defining the selectivity of processes along the auxin response chain: a study using auxin analogues

SYSNO ASEP	0426212
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Defining the selectivity of processes along the auxin response chain: a study using auxin analogues
Author(s)	Simon, Sibu (UEB-Q)_ORCID Kubeš, Martin (UEB-Q)_{RID, ORCID} Baster, P. (BE) Robert, S. (BE) Dobrev, Petre (UEB-Q)_{RID, ORCID} Friml, J. (BE) Petrášek, Jan (UEB-Q)_{RID, ORCID} Zažímalová, Eva (UEB-Q)_{RID, ORCID}
Source Title	New Phytologist - ISSN 0028-646X Roč. 200, č. 4 (2013), s. 1034-1048
Number of pages	15 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	auxin analogues ; auxin signalling ; auxin transport
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GAP305/11/0797 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000330955300012
DOI	10.1111/nph.12437
Annotation	The mode of action of auxin is based on its non-uniform distribution within tissues and organs. Despite the wide use of several auxin analogues in research and agriculture, little is known about the specificity of different auxin-related transport and signalling processes towards these compounds. Using seedlings of Arabidopsis thaliana and suspension-cultured cells of Nicotiana tabacum (BY-2), the physiological activity of several auxin analogues was investigated, together with their capacity to induce auxin-dependent gene expression, to inhibit endocytosis and to be transported across the plasma membrane. This study shows that the specificity criteria for different auxin-related processes vary widely. Notably, the special behaviour of some synthetic auxin analogues suggests that they might be useful tools in investigations of the molecular mechanism of auxin action. Thus, due to their differential stimulatory effects on DR5 expression, indole-3-propionic (IPA) and 2,4,5-trichlorophenoxy acetic (2,4,5-T) acids can serve in studies of TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALLING F-BOX (TIR1/AFB)-mediated auxin signalling, and 5-fluoroindole-3-acetic acid (5-F-IAA) can help to discriminate between transcriptional and non-transcriptional pathways of auxin signalling. The results demonstrate that the major determinants for the auxin-like physiological potential of a particular compound are very complex and involve its chemical and metabolic stability, its ability to distribute in tissues in a polar manner and its activity towards auxin signalling machinery.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2014

Number of the records: 1