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Translation Initiation Factors eIF3 and HCR1 Control Translation Termination and Stop Codon Read-Through in Yeast Cells

SYSNO ASEP	0425640
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Translation Initiation Factors eIF3 and HCR1 Control Translation Termination and Stop Codon Read-Through in Yeast Cells
Author(s)	Beznosková, Petra (MBU-M)_RID Cuchalová, Lucie (MBU-M) Wagner, Susan (MBU-M)_{RID, ORCID} Shoemaker, Ch. J. (US) Gunišová, Stanislava (MBU-M)_RID von der Haar, T. (GB) Valášek, Leoš Shivaya (MBU-M)_{RID, ORCID}
Source Title	PLoS Genetics. - : Public Library of Science - ISSN 1553-7404 Roč. 9, č. 11 (2013)
Number of pages	17 s.
Language	eng - English
Country	US - United States
Keywords	RNA RECOGNITION MOTIF ; MESSENGER-RNA ; IN-VIVO
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GBP305/12/G034 GA ČR - Czech Science Foundation (CSF)
Institutional support	MBU-M - RVO:61388971
UT WOS	000330369000042
DOI	10.1371/journal.pgen.1003962
Annotation	Translation is divided into initiation, elongation, termination and ribosome recycling. Earlier work implicated several eukaryotic initiation factors (eIFs) in ribosomal recycling in vitro. Here, we uncover roles for HCR1 and eIF3 in translation termination in vivo. A substantial proportion of eIF3, HCR1 and eukaryotic release factor 3 (eRF3) but not eIF5 (a well-defined "initiation-specific" binding partner of eIF3) specifically co-sediments with 80S couples isolated from RNase-treated heavy polysomes in an eRF1-dependent manner, indicating the presence of eIF3 and HCR1 on terminating ribosomes. eIF3 and HCR1 also occur in ribosome-and RNA-free complexes with both eRFs and the recycling factor ABCE1/RLI1. Several eIF3 mutations reduce rates of stop codon read-through and genetically interact with mutant eRFs. In contrast, a slow growing deletion of hcr1 increases read-through and accumulates eRF3 in heavy polysomes in a manner suppressible by overexpressed ABCE1/RLI1. Based on these and other findings we propose that upon stop codon recognition, HCR1 promotes eRF3.GDP ejection from the post-termination complexes to allow binding of its interacting partner ABCE1/RLI1
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2014

Number of the records: 1