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The Variability of the 16S rRNA Gene in Bacterial Genomes and Its Consequences for Bacterial Community Analyses

SYSNO ASEP	0423805
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The Variability of the 16S rRNA Gene in Bacterial Genomes and Its Consequences for Bacterial Community Analyses
Author(s)	Větrovský, Tomáš (MBU-M)_{ORCID, RID} Baldrian, Petr (MBU-M)_{RID, ORCID}
Source Title	PLoS ONE. - : Public Library of Science - ISSN 1932-6203 Roč. 8, č. 2 (2013)
Number of pages	10 s.
Language	eng - English
Country	US - United States
Keywords	OPERON COPY NUMBER ; MICROBIAL COMMUNITIES ; MOLECULAR MARKERS
Subject RIV	EE - Microbiology, Virology
R&D Projects	LD12048 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LD12050 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971
UT WOS	000315519000169
DOI	10.1371/journal.pone.0057923
Annotation	16S ribosomal RNA currently represents the most important target of study in bacterial ecology. Its use for the description of bacterial diversity is, however, limited by the presence of variable copy numbers in bacterial genomes and sequence variation within closely related taxa or within a genome. Here we use the information from sequenced bacterial genomes to explore the variability of 16S rRNA sequences and copy numbers at various taxonomic levels and apply it to estimate bacterial genome and DNA abundances. In total, 7,081 16S rRNA sequences were in silico extracted from 1,690 available bacterial genomes (1-15 per genome). While there are several phyla containing low 16S rRNA copy numbers, in certain taxa, e. g., the Firmicutes and Gammaproteobacteria, the variation is large. Genome sizes are more conserved at all tested taxonomic levels than 16S rRNA copy numbers. Only a minority of bacterial genomes harbors identical 16S rRNA gene copies, and sequence diversity increases with increasing copy numbers
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2014

Number of the records: 1