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Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis
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SYSNO ASEP 0421943 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis Author(s) Ranocha, P. (FR)
Dima, O. (BE)
Nagy, R. (CH)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Goffner, D. (FR)Number of authors 18 Source Title Nature Communications. - : Nature Publishing Group
Roč. 4, č. 2013 (2013)Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords BINDING PROTEIN-1 ; POLAR TRANSPORT ; PLANTS Subject RIV EF - Botanics R&D Projects LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000326472200014 DOI 10.1038/ncomms3625 Annotation The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in plant development. Its spatiotemporal distribution is controlled by a combination of biosynthetic, metabolic and transport mechanisms. Four families of auxin transporters have been identified that mediate transport across the plasma or endoplasmic reticulum membrane. Here we report the discovery and the functional characterization of the first vacuolar auxin transporter. We demonstrate that WALLS ARE THIN1 (WAT1), a plant-specific protein that dictates secondary cell wall thickness of wood fibres, facilitates auxin export from isolated Arabidopsis vacuoles in yeast and in Xenopus oocytes. We unambiguously identify IAA and related metabolites in isolated Arabidopsis vacuoles, suggesting a key role for the vacuole in intracellular auxin homoeostasis. Moreover, local auxin application onto wat1 mutant stems restores fibre cell wall thickness. Our study provides new insight into the complexity of auxin transport in plants and a means to dissect auxin function during fibre differentiation. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2014
Number of the records: 1