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Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells

SYSNO ASEP	0421122
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells
Author(s)	Čovanová, Milada (UEB-Q)_{RID, ORCID} Sauer, M. (BE) Rychtář, J. (CA) Friml, J. (DE) Petrášek, Jan (UEB-Q)_{RID, ORCID} Zažímalová, Eva (UEB-Q)_{RID, ORCID}
Source Title	PLoS ONE. - : Public Library of Science - ISSN 1932-6203 Roč. 8, č. 7 (2013)
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	ZEA-MAYS-L ; PLANT HORMONE AUXIN ; MEMBRANE H+-ATPASE
Subject RIV	ED - Physiology
R&D Projects	GAP305/11/0797 GA ČR - Czech Science Foundation (CSF)
	GPP501/12/P951 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000325211000181
DOI	10.1371/journal.pone.0070050
Annotation	Background: Auxin binding protein 1 (ABP1) is a putative auxin receptor and its function is indispensable for plant growth and development. ABP1 has been shown to be involved in auxin-dependent regulation of cell division and expansion, in plasma-membrane-related processes such as changes in transmembrane potential, and in the regulation of clathrin-dependent endocytosis. However, the ABP1-regulated downstream pathway remains elusive. Methodology/Principal Findings: Using auxin transport assays and quantitative analysis of cellular morphology we show that ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can counterbalance increased auxin efflux and auxin starvation phenotypes caused by the overexpression of PIN auxin efflux carrier. Relevant mechanism involves the ABP1-controlled vesicle trafficking processes, including positive regulation of endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery after photobleaching (FRAP) and pharmacological manipulations. Conclusions/Significance: The findings indicate the involvement of ABP1 in control of rate of auxin transport across plasma membrane emphasizing the role of ABP1 in regulation of PIN activity at the plasma membrane, and highlighting the relevance of ABP1 for the formation of developmentally important, PIN-dependent auxin gradients.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2014

Number of the records: 1