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Arabidopsis Exocyst Subcomplex Containing Subunit EXO70B1 Is Involved in Autophagy-Related Transport to the Vacuole

SYSNO ASEP	0421002
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Arabidopsis Exocyst Subcomplex Containing Subunit EXO70B1 Is Involved in Autophagy-Related Transport to the Vacuole
Author(s)	Kulich, Ivan (UEB-Q) Pečenková, Tamara (UEB-Q)_{RID, ORCID} Sekereš, J. (CZ) Smetana, Ondřej (UEB-Q) Fendrych, Matyáš (UEB-Q) Foissner, I. (AT) Hoftberger, M. (AT) Žárský, Viktor (UEB-Q)_{RID, ORCID}
Source Title	Traffic. - : Wiley - ISSN 1398-9219 Roč. 14, č. 11 (2013), s. 1155-1165
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	Arabidopsis ; autophagy ; EXO70
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GAP305/11/1629 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000325482300005
DOI	10.1111/tra.12101
Annotation	Autophagic transport to the vacuole represents an endomembrane trafficking route, which is widely used in plants, not only during stress situations, but also for vacuole biogenesis and during developmental processes. Here we report a role in autophagic membrane transport for EXO70B1one of 23 paralogs of ArabidopsisEXO70 exocyst subunits. EXO70B1 positive compartments are internalized into the central vacuole and co-localize with autophagosomal marker ATG8f. This internalization is boosted by induction of autophagy. Loss of function (LOF) mutations in exo70B1 cause reduction of internalized autopagic bodies in the vacuole. Mutant plants also show ectopic hypersensitive response (HR) mediated by salicylic acid (SA) accumulation, increased nitrogen starvation susceptibility and anthocyanin accumulation defects. Anthocyanin accumulation defect persists in npr1x exo70B1 double mutants with SA signaling compromised, while ectopic HR is suppressed. EXO70B1 interacts with SEC5 and EXO84 and forms an exocyst subcomplex involved in autophagy-related, Golgi-independent membrane traffic to the vacuole. We show that EXO70B1 is functionally completely different from EXO70A1 exocyst subunit and adopted a specific role in autophagic transport.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2014

Number of the records: 1