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Zinc induces DNA damage in tobacco roots
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SYSNO ASEP 0399938 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Zinc induces DNA damage in tobacco roots Author(s) Procházková, Dagmar (UEB-Q) RID
Wilhelmová, Naděžda (UEB-Q) RID, ORCID
Pavlíková, D. (CZ)
Száková, J. (CZ)
Gichner, Tomáš (UEB-Q)Source Title Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134
Roč. 57, č. 4 (2013), s. 783-787Number of pages 5 s. Language eng - English Country NL - Netherlands Keywords comet assay ; ethyl methanesulphonate ; Nicotiana tabacum Subject RIV EF - Botanics R&D Projects GAP501/11/1239 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000324878300025 DOI 10.1007/s10535-013-0345-x Annotation We applied the alkaline version of the single-cell gel electrophoresis (comet assay) to seedlings of heterozygous tobacco (Nicotiana tabacum L. var. xanthi) treated with zinc acetate dihydrate (20 to 80 mM Zn2+ for 2 h or 2 to 12 mM Zn2+ for 24 h). A dose dependent increase in DNA damage expressed by the tail moment values were observed in nuclei isolated from the roots after 2 and 24 h Zn2+ treatments. In contrast, Zn2+ did not induce significant DNA damage to leaf nuclei, with the exception of 10 or 12 mM Zn2+ for 24 h. Somatic mutations, identified as dark green, yellow, and dark green/yellow double sectors on the pale green tobacco leaves were not detected after any Zn2+ treatments. The accumulation of Zn in roots and shoots was determined by inductively coupled plasma optical emission spectrometry and the Zn content in roots was about three times higher than in shoots. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2014
Number of the records: 1