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Cold Response of Dedifferentiated Barley Cells at the Gene Expression, Hormone Composition, and Freezing Tolerance Levels: Studies on Callus Cultures
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SYSNO ASEP 0396875 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cold Response of Dedifferentiated Barley Cells at the Gene Expression, Hormone Composition, and Freezing Tolerance Levels: Studies on Callus Cultures Author(s) Vashegyi, I. (HU)
Marozsan-Toth, Z. (HU)
Galiba, G. (HU)
Dobrev, Petre (UEB-Q) RID, ORCID
Vaňková, Radomíra (UEB-Q) RID, ORCID
Toth, B. (HU)Source Title Molecular Biotechnology. - : Springer - ISSN 1073-6085
Roč. 54, č. 2 (2013), s. 337-349Number of pages 13 s. Language eng - English Country US - United States Keywords ABA ; Barley ; Callus Subject RIV ED - Physiology R&D Projects GA522/09/2058 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000318308400025 DOI 10.1007/s12033-012-9569-9 Annotation In this study, data is presented how dark-grown, embryogenic barley callus cells respond to cold without any light-dependent, chloroplast-related mechanism, independently of the systemic signals. The expression of HvCBF9, HvCBF14, and HvCOR14b genes, members of one of the most important cold-inducible regulatory system, was measured by real-time PCR. Characteristic of the cold response was similar in the crowns of seedlings and in dark-grown callus cultures, however, gene expression levels were lower in calli. Endogenous concentration of auxins, abscisic acid, and salicylic acid did not change, but phaseic acid and neophaseic acid showed robust accumulation after cold acclimation. Freezing tolerance of the cultures was also higher after 7 days of cold-hardening. The results suggest the presence of a basal, light-independent, cold-responsive activation of the CBF-COR14b pathway in barley cultures. The effects of Dicamba, the exogenous auxin analog used for maintaining tissue cultures were also studied. Dicamba seems to be a general enhancer of the gene expression and physiological responses to cold stress, but has no specific effect on the activation. Our data along with previous findings show that this system might be a suitable model for studying certain basic cellular mechanisms involved in the cold acclimation process in cereals. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2014
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