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Expression of a recombinant Human papillomavirus 16 E6GT oncoprotein fused to N- and C-termini of Potato virus X coat protein in Nicotiana benthamiana
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SYSNO ASEP 0396719 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Expression of a recombinant Human papillomavirus 16 E6GT oncoprotein fused to N- and C-termini of Potato virus X coat protein in Nicotiana benthamiana Author(s) Čeřovská, Noemi (UEB-Q) RID, ORCID
Moravec, Tomáš (UEB-Q) RID, ORCID
Hoffmeisterová, Hana (UEB-Q) RID
Plchová, Helena (UEB-Q) RID, ORCID
Synková, Helena (UEB-Q) RID, ORCID
Poláková, I. (CZ)
Dušková, M. (CZ)
Šmahel, M. (CZ)Source Title Plant Cell, Tissue and Organ Culture. - : Springer - ISSN 0167-6857
Roč. 113, č. 1 (2013), s. 81-90Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords Transient expression ; Molecular farming ; Potato virus X Subject RIV EE - Microbiology, Virology R&D Projects GA521/09/1525 GA ČR - Czech Science Foundation (CSF) GAP501/12/1761 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000316761000008 DOI 10.1007/s11240-012-0253-3 Annotation Infection with Human papillomaviruses (HPVs) is linked to cervical cancer, which is one of the most common cancers found among women worldwide. Despite preventive immunization, a therapeutic vaccine, targeting infected individuals, is required. Vaccine strategies for treatment of HPV-induced cervical cancer are based on E7 and E6 oncoproteins. In this work we report the transient expression of chimeric particles containing the E6 oncoprotein from Human papillomavirus type 16 (HPV-16) in plants. We fused a mutagenized coding sequence of the E6 oncoprotein (E6GT) with the coding sequence of Potato virus X coat protein (PVX CP) both with the 5'- and 3'-terminus using linkers of different length (0, 4 or 15 amino acids). The expression in E. coli was performed to assess the characteristics of the recombinant protein prior to plant expression. The yield and immunological reactivity of the expressed proteins were screened with anti-PVX CP and E6 antibodies. The highest yields of chimeric particles were observed in the transgenic N. benthamiana expressing Potato virus A HC-Pro protein and the Tobacco mosaic virus movement protein. When inoculated on host plants, these recombinant viruses were not able to spread systemically. The obtained results revealed the new relations for design of expression cassettes for plant-based vaccine production. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2014
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