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Corynebacterium glutamicum promoters: a practical approach
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SYSNO ASEP 0396036 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Corynebacterium glutamicum promoters: a practical approach Author(s) Pátek, Miroslav (MBU-M) RID, ORCID
Holátko, Jiří (MBU-M)
Busche, T. (DE)
Kalinowski, J. (DE)
Nešvera, Jan (MBU-M) RIDSource Title Microbial Biotechnology - ISSN 1751-7907
Roč. 6, č. 2 (2013), s. 103-117Number of pages 15 s. Language eng - English Country GB - United Kingdom Keywords VITRO TRANSCRIPTION SYSTEM ; L-LYSINE PRODUCTION ; SIGMA-FACTOR SIGB Subject RIV EE - Microbiology, Virology R&D Projects GPP302/12/P633 GA ČR - Czech Science Foundation (CSF) Institutional support MBU-M - RVO:61388971 UT WOS 000315124400002 DOI 10.1111/1751-7915.12019 Annotation Transcription initiation is the key step in gene expression in bacteria, and it is therefore studied for both theoretical and practical reasons. Promoters, the traffic lights of transcription initiation, are used as construction elements in biotechnological efforts to coordinate green waves' in the metabolic pathways leading to the desired metabolites. Detailed analyses of Corynebacterium glutamicum promoters have already provided large amounts of data on their structures, regulatory mechanisms and practical capabilities in metabolic engineering. In this minireview the main aspects of promoter studies, the methods developed for their analysis and their practical use in C.glutamicum are discussed. These include definitions of the consensus sequences of the distinct promoter classes, promoter localization and characterization, activity measurements, the functions of transcriptional regulators and examples of practical uses of constitutive, inducible and modified promoters in biotechnology. The implications of the introduction of novel techniques, such as in vitro transcription and RNA sequencing, to C.glutamicum promoter studies are outlined Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2014
Number of the records: 1