Number of the records: 1
Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato
- 1.
SYSNO ASEP 0395286 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato Author(s) Kodrík, Dalibor (BC-A) RID, ORCID
Kludkiewicz, Barbara (BC-A) ORCID
Navrátil, Oldřich (UEB-Q) RID, ORCID
Skoková Habuštová, Oxana (BC-A) RID
Horáčková, V. (CZ)
Svobodová, Z. (CZ)
Vinokurov, Konstantin (BC-A) RID, ORCID
Sehnal, František (BC-A) RIDNumber of authors 8 Source Title Applied Biochemistry and Biotechnology. - : Springer - ISSN 0273-2289
Roč. 171, č. 1 (2013), s. 209-224Number of pages 16 s. Language eng - English Country US - United States Keywords genetically modified organism ; silk protease inhibitor ; protease Subject RIV ED - Physiology R&D Projects QI91A229 GA MZe - Ministry of Agriculture (MZe) Institutional support BC-A - RVO:60077344 ; UEB-Q - RVO:61389030 UT WOS 000324109800018 EID SCOPUS 84883801105 DOI 10.1007/s12010-013-0325-9 Annotation Several recombinant derivatives of silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subtilisin and proteinase K. A construct of the gene was introduced into Agrobacterium tumefaciens that was then used for genetic transformation of the potato variety Velox. In vitro tests showed increased resistance to the late blight Phytophthora infestans in several transformed lines. No effect was seen on biological characteristics of Spodoptera littoralis indicating that the transformed potatoes may be harmless to non-target organisms. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2014 Electronic address http://link.springer.com/content/pdf/10.1007%2Fs12010-013-0325-9.pdf
Number of the records: 1