Number of the records: 1

Molecular Analysis and Genomic Organization of Major DNA Satellites in Banana (Musa spp.)

SYSNO ASEP	0394043
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Molecular Analysis and Genomic Organization of Major DNA Satellites in Banana (Musa spp.)
Author(s)	Čížková, Jana (UEB-Q)_{RID, ORCID} Hřibová, Eva (UEB-Q)_{RID, ORCID} Humplíková, Lenka (UEB-Q) Christelová, Pavla (UEB-Q)_{RID, ORCID} Suchánková, Pavla (UEB-Q)_RID Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Source Title	PLoS ONE. - : Public Library of Science - ISSN 1932-6203 Roč. 8, č. 1 (2013)
Number of pages	14 s.
Language	eng - English
Country	US - United States
Keywords	IN-SITU HYBRIDIZATION ; LARGE-SCALE ORGANIZATION ; RIBOSOMAL-RNA GENES
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	KJB500380901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LG12021 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000314021500130
DOI	10.1371/journal.pone.0054808
Annotation	Satellite DNA sequences consist of tandemly arranged repetitive units up to thousands nucleotides long in head-to-tail orientation. The evolutionary processes by which satellites arise and evolve include unequal crossing over, gene conversion, transposition and extra chromosomal circular DNA formation. Large blocks of satellite DNA are often observed in heterochromatic regions of chromosomes and are a typical component of centromeric and telomeric regions. Satellite-rich loci may show specific banding patterns and facilitate chromosome identification and analysis of structural chromosome changes. Unlike many other genomes, nuclear genomes of banana (Musa spp.) are poor in satellite DNA and the information on this class of DNA remains limited. The banana cultivars are seed sterile clones originating mostly from natural intra-specific crosses within M. acuminata (A genome) and inter-specific crosses between M. acuminata and M. balbisiana (B genome). Previous studies revealed the closely related nature of the A and B genomes, including similarities in repetitive DNA. In this study we focused on two main banana DNA satellites, which were previously identified in silico. Their genomic organization and molecular diversity was analyzed in a set of nineteen Musa accessions, including representatives of A, B and S (M. schizocarpa) genomes and their inter-specific hybrids. The two DNA satellites showed a high level of sequence conservation within, and a high homology between Musa species. FISH with probes for the satellite DNA sequences, rRNA genes and a single-copy BAC clone 2G17 resulted in characteristic chromosome banding patterns in M. acuminata and M. balbisiana which may aid in determining genomic constitution in interspecific hybrids. In addition to improving the knowledge on Musa satellite DNA, our study increases the number of cytogenetic markers and the number of individual chromosomes, which can be identified in Musa.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2014

Number of the records: 1