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Endogenous cytokinin profiles and their relationships to between-family differences during adventitious caulogenesis in Pinus pinea cotyledons
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SYSNO ASEP 0389141 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Endogenous cytokinin profiles and their relationships to between-family differences during adventitious caulogenesis in Pinus pinea cotyledons Author(s) Cuesta, C. (ES)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Ordas, R. J. (ES)
Fernandez, B. (ES)
Strnad, Miroslav (UEB-Q) RID, ORCID
Doležal, Karel (UEB-Q) RID, ORCID
Rodriguez, A. (ES)Source Title Journal of Plant Physiology. - : Elsevier - ISSN 0176-1617
Roč. 169, č. 18 (2012), s. 1830-1830-1837Number of pages 8 s. Language eng - English Country PT - Portugal Keywords Natural cytokinins ; Adventitious shoot organogenesis ; Pinus pinea Subject RIV EC - Immunology R&D Projects KAN200380801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000313389800006 DOI 10.1016/j.jplph.2012.08.012 Annotation Caulogenesis in mature stone pine (Pinus pinea L.) cotyledons is promoted, to varying degrees depending on genotype, by exogenous application of the cytokinin (CK) benzyladenine (BA). In the present study, endogenous CK profiles of cotyledons from open-pollinated plants and two families of stone pine with widely differing organogenic capacities were monitored during caulogenesis and linked to previously characterized BA uptake and induction phases. Changes in levels of free bases, ribosides, ribotides and glucosides of both isoprenoid and aromatic CKs were followed. Before BA application, the pool of endogenous CKs in all sets of cotyledons was dominated by isoprenoid ribotides, but 1 h after BA exposure, aromatic CKs (mainly active free bases and ribosides of topolins) accounted for more than 90% of the pool. BA N-glucosides were also observed, levels of which (and topolins) rose from 2 d until the end of the (six-day) culture period. The CK profiles of the two selected pine families also differed, although the general trends were similar. During the first 6 h, levels of BA and meta-topolin were highest in cotyledons from the family with the strongest caulogenic responses, while levels of ribotides and aromatic glucosides were highest in cotyledons from the other family. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2013
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